抗纤益肺胶囊对肺纤维化大鼠肺组织病理变化及血清TGF-β1、TNF-α的影响  

Influences of Kangxian Yifei Capsules on lung histopathological changes and serum TGF-β1 and TNF-α in rats with pulmonary fibrosis

作　　者：赵勤萍[1] 王洋[1] 薛晓明[1] 关炜[1] 

机构地区：[1]山西省中医院,山西030012

出　　处：《北京中医药大学学报（中医临床版）》2012年第6期16-19,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：山西省科技攻关项目(No.20100311082-3)

摘　　要：目的观察抗纤益肺胶囊对博莱霉素致大鼠肺纤维化的病理改变及血清肿瘤坏死因子-α(TNF-α)、转化生长因子-β1(TGF-β1)的影响。方法选用60只健康Wistar大鼠,随机分为正常组,模型组,阳性对照组,抗纤益肺胶囊高、中、低剂量组,采用经气管滴注博莱霉素溶液建立肺纤维化大鼠模型。在造模48h后,各组分别予生理盐水,泼尼松混悬液,抗纤益肺胶囊高、中、低剂量药物,每日1次,共28d。观察大鼠肺组织形态学变化,以及对肺系数测定和肺组织羟脯氨酸(HYP)、血清TGF-β1及TNF-α的影响。结果与模型组比较,抗纤益肺胶囊各组和阳性对照组的病理形态学变化明显减轻,随着抗纤益肺胶囊剂量增加,肺纤维化程度降低。抗纤益肺胶囊各组HYP、TGF-β1和TNF-α均明显低于模型组(P<0.05),而且随着剂量增加,降低更明显。与阳性对照组比较,抗纤益肺胶囊高剂量组HYP有明显降低(P<0.05)。结论抑制肺组织中TGF-β1和TNF-α的表达,以及抑制胶原蛋白合成,可能是抗纤益肺胶囊抗肺纤维化作用的机制之一。Objective To observe the influences of Kangxian Yifei Capsules on lung histopathological changes and serum tumor necrosis factor-α （TNF-α and transforming growth factor-β1 （TGF-β1） in rats with pulmonary fibrosis induced by bleomycin. Methods Health Wistar rats （n=60） were randomly divided into normal group, model group, positive control group, and high-dose, mid-dose and low-dose Kangxian Yifei Capsules groups （high-dose group, mid-dose group and low-dose group）. The rat model of pulmonary fibrosis was established by tracheal instillation of bleomycin solution, and after 48 hours all groups were respectively given normal saline solution, prednisone suspension, and high-dose, mid-dose and low-dose Kangxian Yifei Capsules once a day for 28 days. The histomorphological changes of lung, lung coefficient, hydroxyproline （HYP） in lung tissue and serum TNF-α and TGF-β1 were observed and detected. Results Compared with model group, the histomorphological changes of lung were relieved significantly in all groups of Kangxian Yifei Capsules and positive control group. Along with the increase of dose of Kangxian Yifei Capsules, the degree of pulmonary fibrosis decreased. HYP, TNF-α and TGF-β1 were significantly lower in all groups of Kangxian Yifei Capsules than those in model group （P〈0.05）, which was more significant as the increases of dose. Compared with positive control group, HYP decreased significantly in high-dose group （P〈0.05）. Conclusion The inhibition of the expressions of TNF-α and TGF-β1 and collagen synthesis may be one of anti-fibrosis mechanisms of Kangxian Yifei Capsules.

关 键 词：抗纤益肺胶囊 肺纤维化 大鼠 血清肿瘤坏死因子 转化生长因子 

分 类 号：R256.1[医药卫生—中医内科学]