注射用黄芪多糖对耐顺铂人肺腺癌细胞A549/DDP耐药逆转作用研究  

Resistance reversal effect of astragalus polysaccharides for injection on cisplatin-resistant human lung adenocarcinoma cell line A549/DDP

作　　者：张莹[1] 贾英杰[1] 李小江[1] 朱津丽[1] 张欣[1] 张丽丽[1] 

机构地区：[1]天津中医药大学第一附属医院肿瘤科,天津300193

出　　处：《药物评价研究》2012年第6期417-419,共3页Drug Evaluation Research

基　　金：天津市应用基础及前沿技术研究计划(青年基金项目)(12JCQNJC07500);天津市高等学校科技发展基金计划项目(20110223)

摘　　要：目的探讨注射用黄芪多糖药物血清在体外对耐顺铂(DDP)人肺腺癌A549/DDP细胞株的耐药逆转作用。方法通过建立小鼠肿瘤模型,运用血清药理学方法,以人肺腺癌敏感细胞A549和耐药细胞A549/DDP为研究对象,采用细胞毒实验(MTT)方法,探讨注射用黄芪多糖对耐顺铂人肺腺癌细胞A549/DDP的耐药逆转作用。结果注射用黄芪多糖药物血清高、中、低剂量组分别与DDP合用时对A549/DDP细胞的耐药逆转倍数分别为1.24、1.41、1.34;且中、高剂量组与阳性对照DDP组比较差异有统计学意义(P<0.05)。结论注射用黄芪多糖能明显增加A549/DDP耐药细胞对DDP的敏感性,提高细胞死亡率,具有耐药逆转作用。Objective To investigate the resistance reversal effect of the drug serum of astragalus polysaccharides for injection（API） in vitro on cisplatin-resistant human lung adenocarcinoma cell line A549/DDP.Methods The model of tumor-bearing mice was established,and using serum pharmacology method and taking human lung adenocarcinoma sensitive cell A549 and drug-resistant cell A549/DDP as the research objects,the resistance reversal effect of API on cisplatin-resistant human adenocarcinoma cells A549/DDP was studied in cell toxicity experiment by MTT method.Results The resistance reversal multiples of high-,mid-,and low-dose groups of API drug serum to A549/DDP cells were 1.24,1.41,and 1.34,respectively;The data in mid-and high-dose groups had significant difference（P0.05） compared with DDP（positive control） group.Conclusion API could obviously increase the sensitivity of A549/DDP cells on DDP and improve the cell mortality.API has the resistance reversal effect.

关 键 词：注射用黄芪多糖 肺癌 A549 DDP 耐药性 

分 类 号：R979.1[医药卫生—药品]