孔径及壳聚糖对多孔β-TCP中庆大霉素释放的影响  

Role of Pore Size and Chitosan in Gentamicin Release from Porous β-TCP:in Vitro Study

作　　者：刘楠[1] 赵黎[1] 胡玲珑[1] 李海[1] 

机构地区：[1]上海交通大学医学院附属新华医院,上海200092

出　　处：《现代生物医学进展》2012年第32期6207-6213,共7页Progress in Modern Biomedicine

基　　金：教育部高等学校博士学科点新教师基金课题(20090073120037);上海市科委医学引导类项目(09411962600);上海市卫生局青年科研项目(2008Y069)

摘　　要：目的:观察不同孔径的多孔β-TCP分别复合庆大霉素及庆大霉素/壳聚糖后在体外庆大霉素的释放情况,研究多孔β-TCP孔径及壳聚糖对庆大霉素释放的影响。方法:利用冷冻干燥法使不同孔径的多孔β-TCP分别复合庆大霉素及庆大霉素/壳聚糖。比色法测定样本液内硫酸庆大霉素浓度,抑菌环实验测定样本液中庆大霉素的抗菌性。结果:(1)多孔β-TCP/庆大霉素中庆大霉素的释放时间分别为72h(187-300μm)、48h(300-375μm)、48h(375-500μm)、48h(500-750μm)、48h(750-830μm),孔径187-300μm组释放时间最长。(2)多孔β-TCP/庆大霉素/壳聚糖样本中庆大霉素的释放时间分别为12d(187-300μm)、12d(300-375μm)、12d(375-500μm)、13d(500-750μm)、12d(750-830μm),较β-TCP/庆大霉素中时间长,且在500-750μm上作用最显著。结论:(1)孔径对于庆大霉素释放具有一定的影响作用,187-300μm是延长多孔β-TCP/庆大霉素释放的最适孔径(在187-830μm内),但是时间太短。(2)壳聚糖能够显著延长庆大霉素在β-TCP上的释放时间,而在500-750μm孔径上作用最强。Objective： To investigate the release of gentamicin from porous β-tricalcium phosphate （TCP）-gentamicin of various pore sizes from porous β-TCP-gentamicin-chitosan in vitro and to evaluate the effect of sizes and chitosan on the antibiotics release. Methods： Gentamicin and gentamicin-chitosan was loaded into β-TCP of various pore sizes by freeze-drying. The gentamicin concentrati-on of each sample was measured as the Zhang＇s method and the bioactivity of gentamicin in each sample was measured by the assay of inhibition zone. Results： （1）The release of gentamicin from porous β-TCP were sustained for 72 hours （187-300μm）, 48 hours （300-375μm）, 48 hours （375-500μm） , 48 hours （500-750pμm） and 48 hours （750-830μm）respectively, while among them, the longest duration of release was found in the group with pore size of 187-300μm. （2） The duration of released gentamicin in β-TCP-chitosan-gen-tamicin were respectively 12 days （187-300μm）, 12days （300-375μm）, 12days （375-500μm）, βdays （500-750μm） and 12days （750-830 μm）. These were longer than duration in β-TCP-gentamicin. The longest duration was found in the group with pore size of 500-750μm. Coiielusions ：（1） Pore size of β-TCP may play a certain role in controlling the delivery of antibiotics loaded. The pore size of 187-300μm was the most appropriate pore size （range of pore size： 187-830μm） for prolonging gentamicin release loaded into porous β-TCP, but the duration was too short. （2） The chitosan could significantly prolong the gentamicin release loaded into porous β-TCP. The most powerful carder was in the pore size of 500-750μm.

关 键 词：药物缓释 硫酸庆大霉素 Β-TCP 壳聚糖 孔径 

分 类 号：R68[医药卫生—骨科学] R318.08[医药卫生—外科学]