HPLC法测定丹参饮片中丹参酮ⅡA的含量  

Determination of Tanshinone ⅡA in Salvia miltiorrhiza Bunge by HPLC

作　　者：蒲清荣[1] 陈玉兰[1] 赵剑[1] 李能源[1] 颜章龙[1] 

机构地区：[1]泸州医学院附属中医医院,四川泸州646002

出　　处：《现代生物医学进展》2012年第33期6561-6563,6567,共4页Progress in Modern Biomedicine

摘　　要：目的:采用高效液相色谱法测定市售八批丹参饮片中丹参酮ⅡA的含量。方法:高效液相色谱法,Inertsill ODS-SP(4.6×150mm,5μm),流动相:甲醇-水(75:25),检测波长:270 nm,流速:1.0 ml/min。结果:丹参酮ⅡA在(1.43～68.35)μg/ml范围内有良好的线性关系,r=0.9999(n=6)。丹参饮片中丹参酮ⅡA的含量普遍偏低,只有山东饮片中的两个批次含量较高。结论:考察分析丹参饮片中丹参酮ⅡA的含量,可为临床使用提供依据。Objective： To investigate the content variety of tanshinone II A in Salvia miltiorrhiza Bge in current market. Methods： HPLC was adopted with Inertsill ODS-SP （4.6×150mm,5μm）.The mobile phase consisted of methanol-water （75：25） with a flow of 1.0ml/min at 270nm. Results： The tanshinone Ⅱ A showed good linear relationship in range of 1.43-68.35 μg/ml, r=0.9999 （n=6）. In general,the content of tanshinone Ⅱ A in Salvia miltiorrhiza Bge is comparatively low. The content of tanshinone Ⅱ A in drug of Danshen from Shandong is highest. Conclusions： This paper measures and analyzes the content of tanshinone Ⅱ A in Salvia miltiohiza Bunge, which will provide a reference for clinical use of Salvia miltiorrhiza Bunge.

关 键 词：丹参 丹参酮ⅡA HPLC 

分 类 号：R284.4[医药卫生—中药学]