利用与大豆灰斑病抗性基因连锁的SSR标记构建大豆品种(系)的分子身份证  

Establishment of Molecular ID of Soybean Varieties (Lines) Using SSR Markers Linked to Resistance Genes against Cercospora sojina

作　　者：丁俊杰[1] 姜翠兰[2] 顾鑫[1] 杨晓贺[1] 赵海红[1] 申宏波[1] 仕相林[3] 刘春燕 胡国华 陈庆山[3] 

机构地区：[1]黑龙江省农业科学院佳木斯分院/农业部佳木斯作物有害生物科学观测实验站,黑龙江佳木斯154007 [2]黑龙江农垦科学院农作物开发研究所,黑龙江佳木斯154007 [3]东北农业大学农学院,黑龙江哈尔滨150030 [4]黑龙江农垦科研育种中心,黑龙江哈尔滨150030

出　　处：《作物学报》2012年第12期2206-2216,共11页Acta Agronomica Sinica

基　　金：黑龙江省自然科学基金项目(C200936);国家公益性行业(农业)科研专项(201103016-03A2);国家现代农业产业技术体系建设佳木斯综合试验站项目资助

摘　　要：以黑龙江省29个大豆育种单位的103份已鉴定大豆灰斑病3个生理小种抗性的大豆品种（系）为材料,选择与大豆灰斑病抗病基因连锁的19个SSR标记检测,获得等位变异数86个,每个标记检测到的等位变异数分布在2~6个之间,平均为4.4个。应用遗传统计软件（Genetics Statistics 3.0）分析表明,标记的多样性指数介于0.198~0.751之间,平均多样性指数为0.606。品种（系）特异指数差异较大,介于46.592~481.541之间,平均为87.415。根据标记的等位基因数,使用ID Analysis1.0软件分析表明,利用与大豆抗灰斑病基因连锁的7个SSR标记（Satt565、Satt547、Satt431、Sct_186、SOYGPATR、Satt244、Sat_151）就能有效区分各品种（系）,因此利用这7个标记构建了供试品种（系）的分子身份证。One hundred and three soybean varieties （lines） developed by 29 breeding units from six accumulated temperature zones in Heilongjiang were investigated. The resistances of 103 soybean varieties （lines） were identified with three races of Cercospora sonija. Eighty-six polymorphic alleles were detected with 19 SSR markers, for each primer, two to six allele variations were detected in all varieties （lines）, with an average of 4.42. The diversity index of the 19 SSR loci ranged from 0.198 to 0.751 with an average of 0.606. The results of calculating specific index and genetic similarity among varieties （lines） showed that specific index of varieties （lines） ranged from 46.592 to 481.541, and the average was 87.415. According to fragment size of allele variation, the data calculated from the PAGE bands were analyzed by the software ID Analysis 1.0. Seven markers （Satt565, Satt547, Satt431, Sct_186, SOYGPATR, Satt244, and Sat_151） linked to the gene controlling resistance to Cercospora sojina were used to identify all 103 soybean lines, so that a set of molecular ID of soybean varieties （lines） was established.

关 键 词：大豆灰斑病 生理小种 SSR 分子身份证 

分 类 号：S565.1[农业科学—作物学]