营养性贫血患者MCL-1和BAK蛋白的表达及其临床意义的研究  

Expression of MCL-1 and BAK Proteins in Nutritional Anemia and Its Clinical Significance

作　　者：郭玉洁[1] 王艳[1] 林凤茹[1] 

机构地区：[1]河北医科大学第二医院血液科,河北石家庄050000

出　　处：《中国实验血液学杂志》2012年第6期1419-1422,共4页Journal of Experimental Hematology

摘　　要：本研究旨在探讨MCL-1、BAK蛋白与营养性贫血发生发展的关系及其临床意义。采用酶联免疫吸附双抗体夹心法(ELISA)检测66例营养性贫血患者血清中MCL-1、BAK蛋白表达水平。以18例健康体检者为正常对照。结果表明:①营养性贫血组MCL-1蛋白表达水平明显于低正常对照组(P<0.001),BAK蛋白表达水平明显高于正常对照组(P<0.001),不同类型营养性贫血(缺铁性贫血、巨幼细胞性贫血、混合性贫血)各组间MCL-1、BAK蛋白表达水平无明显差异(P>0.05);②营养性贫血组治疗后MCL-1蛋白表达水平明显高于治疗前(P<0.05),BAK蛋白表达水平明显低于治疗前(P<0.05);③营养性贫血患者MCL-1蛋白与BAK蛋白表达水平呈负相关(r=-0.858,P<0.05)。结论:MCL-1和BAK蛋白在营养性贫血的发生发展中均有重要作用,并可作为协助诊断营养性贫血及判断预后的参考指标。This study was aimed to investiage the relation of MCL-1 and BAK proteins with incidence and development of nutritional anemia （NA） and their clinical significance. The MCL-1 and BAK protein levels in serum of 66 patients with NA were determined by using ELISA. Eighteen healthy people were randomly selected as normal controls. The results indicated that： （1）as compared with normal control group, the expression level of MCL-1 protein in 3 NA groups （ irorn-deficiency anemia, macrocytic anemia, mixed anemia） significantly decreased （ P 〈 0.001 ）, while the expression level of BAK protein obviously increased （P 〈 0.001 ）, but the expression level of MCL-1 and BAK proteins among 3 NA groups showed no obvious differences ; （ 2 ） the MCL-1 protein expression level increased and BAK protein expression level decreased in 3 NA groups after treatment （ P 〈 0.05 ）. （ 3 ） there was negative correlation of expression levels of MCL-1 protein with BAK protein in NA group （ r = - 0. 858 P 〈 0.05 ）. It is concluded that the MCL-1 and BAK proteins may play an important role in the incidence and development of NA, and can be used as the assist index for defining diagnosis and evaluate prognosis of NA.

关 键 词：营养性贫血 细胞凋亡 MCL-1蛋白 BAK蛋白 

分 类 号：R556.2[医药卫生—血液循环系统疾病]