实时荧光定量PCR快速诊断肠道病毒71型感染  

Real-time fluorescence quantitative PCR in the rapid molecular diagnosis of enterovirus 71 infection

作　　者：许东亮[1] 聂晓晶[2] 张国成[1] 钱新宏[1] 杨晓蕾[1] 邓军霞[1] 李玲莉[1] 

机构地区：[1]第四军医大学西京医院儿科,西安市710032 [2]南京军区福州总医院儿科,福州市350025

出　　处：《中华实用诊断与治疗杂志》2012年第12期1158-1159,共2页Journal of Chinese Practical Diagnosis and Therapy

基　　金：国家自然科学基金(81070543);南京军区医学科技创新课题(09MB135)

摘　　要：目的探寻快速准确早期诊断肠道病毒71型(human enterovirus 71,EV71)感染的方法。方法手足口病患儿871例,采用实时荧光定量PCR检测患儿咽拭子或疱液的EV71感染情况,实时荧光定量PCR检测EV71阳性患儿采用ELISA法检测血清EV71-IgM和EV71-IgG。结果871份标本中,RT-PCR检测EV71感染阳性率为17.91%,其中5月份检测阳性率(24.44%)最高,12月份检测阳性率(4.17%)最低;随机抽取64例RT-PCR检测阳性患儿外周血,EV71-IgM阳性率为51.56%,EV71-IgG阳性率为14.06%。结论 RT-PCR能快速准确诊断EV71早期感染。Objective To explore the rapid molecular diagnosis of enterovirus 71 （EV71） infection. Methods A total of 871 cases of hand foot-and-mouth disease were detected EV71 infection in vesicle fluid and throat swabs with real- time fluorescence quantitative PCR （RT PCR）. The patients with RT PCR comfirmed EV71 infection were detected EVT1-IgM and EV71 IgG with ELISA. Results In these 871 cases, the positive rate of EV71 infection was 17.91%, and it was highest in May （24.44 % ）, and lowest in December （4.17 % ）. Sixty four patients with RT-PCR confirmed EV 71 infection were randomly chosen and detected EV71-IgM and EVT1 IgG with ELISA, with EVTI-IgM positive rate of 51.56%, and EVTI-IgG positive rate of 14.06%. Conclusion RT PCR is an accurate method for quickly detection of EV71.

关 键 词：肠道病毒71型 实时荧光定量PCR 手足口病 

分 类 号：R725.1[医药卫生—儿科]