去细胞组织工程猪肺动脉带瓣管道的实验研究  

Experimental study on decellularized porcine tissue-engineering pulmonary valved conduits

作　　者：王圣[1] 李温斌[1] 武喜红[1] 马金辉[1] 程兆云[1] 赵子牛[1] 

机构地区：[1]河南省人民医院心血管外科,郑州市450003

出　　处：《中华实用诊断与治疗杂志》2012年第12期1191-1194,共4页Journal of Chinese Practical Diagnosis and Therapy

摘　　要：目的探讨去细胞组织工程猪肺动脉带瓣管道的生物特性。方法新鲜猪肺动脉带瓣管道组织3份,A组为对照组,不再进行进一步处理,B组采用胰蛋白酶+Triton X-100去细胞方法对新鲜猪肺动脉带瓣管道进行去细胞处理,C组去细胞处理方法同B组,后行明胶嵌合再处理;3组均采用组织病理HE染色、ETVG染色、免疫组织化学染色及扫描电镜检查,观察去细胞效果和胶原纤维、弹力纤维改变;采用抗胶原纤维抗体间接免疫荧光染色法及扫描电镜、透射电镜检测明胶分子的填充和包裹效果。结果采用去细胞方法可完整去除瓣膜、管壁组织中所有细胞及细胞外基质成分(蛋白多糖、糖蛋白),无细胞核碎片,B组瓣膜及血管壁胶原纤维和弹力纤维呈波浪状整齐排列、结构完整,得到"纯净"猪肺动脉纤维支架;C组可见明胶分子均匀填充在纤维间隙并包裹在纤维支架表面,通过透射电镜初步观察到填充的明胶成分有较好的包裹纤维的作用。结论采用新型方法构建的组织工程猪肺动脉带瓣管道是切实可行的。Objective To study the biological features of decellularized porcine tissue-engineering pulmonary valved conduits. Methods Three fresh porcine pulmonary valved conduits were divided into three groups. Group A was control group. Group B and C were decellularized with trypsin＋ Triton X-100. Group C was retreated with gelatin mosaicing. Pathological HE staining, ETVG staining, immunohistochemical staining and scanning electron microscopy were used to observe decellular effect, collagen fibers and elastic fibers. Anti-collagen antibodies indirect immunofluorescence staining and transmission electron microscopy were used to observe the filling and wrapping effect of the gelatin molecules. Results In group B, there were no cells, extracellular matrix components （proteoglycans and glycoprotein） or nuleus fragmentation. Valves and vessel wall collagen fibers and elastic fibers arranged in neat rows. In group C, gelatin molecules filled in the gaps between fibers and wrapped in the surface of the fiber scaffold uniformly. Conclusion The new method building tissue engineering porcine pulmonary valved conduit is feasible.

关 键 词：肺动脉带瓣管道 组织工程 去细胞 

分 类 号：R318.08[医药卫生—生物医学工程]