黑潮源区沉积物古菌多样性调查及研究  

Diversity of Archaea in the Sediments from the Source Area of Kuroshio

作　　者：景天爽[1,2] 于心科[1] 李铁刚[1] 萨仁高娃[1] 杨再宝[1,2] 

机构地区：[1]中国科学院海洋研究所海洋地质与环境重点实验室,山东青岛266071 [2]中国科学院研究生院,北京100049

出　　处：《地质学报》2012年第12期2011-2019,共9页Acta Geologica Sinica

基　　金：中国科学院知识创新工程重要方向项目(编号YW-JC201;KZCX2-YW-155)资助的成果

摘　　要：为了研究西太平洋黑潮源区不同深度沉积物中古菌群落结构的多样性,应用16SrDNA文库技术,对IMAGES XIV航次采集的岩芯MD06-3047进行基因文库的构建和分析,得到260个克隆,经分析处理后得到56个OTUs(Operational Taxonomic Units)。基于16SrRNA序列的同源性比较,绘制系统进化树,进行统计学分析。研究结果显示,西太平洋黑潮源区的本哈姆高原(Benham Rise)沉积物中有丰富多样的古菌群落,在1m内的沉积物中分布着9种古菌类群。古菌序列归属于泉古菌(Crenarchaeota)和广古菌(Euryarchaeota),并以泉古菌为主(占总序列的91.5%)。其中,泉古菌包含MGI、MBG-B、MCG和UCII-b4个类群,并且主要由MGI构成(占总序列的40.8%),而广古菌包含MBG-D、SAGMEG、DHVEG、MBG-E和VALIII5个类群,各类群所占总序列比例均较低(占总序列的0.4%～5.4%之间)。研究结果对于理解西太平洋黑潮源区沉积物中古菌多样性有重要意义,并为今后研究古菌群落与环境之间的关系提供科学依据。To study the archaeal diversity of deep-sea sediments from the Source Area of Kuroshio in Western Pacific, the MD06-3047 sediment core collected by the IMAGES XIV cruise was used to built a genetic base through 16S rDNAanalysis. 260 archaeal 16S rDNA clones were obtained and after examination, a total of 56 OTUs were yielded. Phylogenetic results show that the sediments from Benham Rise contain a variety of thearchaea and nine groups have been identified within 1 meter of sediments. Archaea belongs to Crenarchaeota and Euryarchaeota, with Crenarchaeota accounting for 91. 5% of total archaeal clones. Crenarchaeota consists of four groups （MGI, MBG-B, MCG and UCII-b）, and is dominated by MGI （40.8% of total archaeal clones）. And Euryarchaeota containsfive archaealphylotypes, which all take up tiny percentage （0. 4% ~ 5. 4%） of total archaeal clones. The results have important implications for understanding of archaeal community in deep-sea sediments from the source area of Kuroshio in Western Pacific and provide a scientific basis for the future study on the relationship between archaea and environment.

关 键 词：西太平洋黑潮源区 沉积物 古菌多样性 16S rDNA文库 

分 类 号：P736.22[天文地球—海洋地质]