肌肽对高糖环境下心肌成纤维细胞增殖的影响及机制  

Effect of carnosine on cell proliferation in rat cardiac fibroblasts induced by high glucose and its mechanism

作　　者：毕惠梅[1] 师岩[2] 崔云霞[1] 闫俊霞[1] 王利华 周宏博[1] 

机构地区：[1]哈尔滨医科大学生物化学与分子生物学教研室,黑龙江哈尔滨150081 [2]齐齐哈尔医学院生物化学教研室,黑龙江齐齐哈尔161006 [3]黑龙江省传染病防治院呼吸二科,黑龙江哈尔滨150500

出　　处：《哈尔滨医科大学学报》2012年第6期526-529,共4页Journal of Harbin Medical University

基　　金：黑龙江省研究生创新科研资金(YJSCX2011-345HLJ);教育部黑龙江省部共建心肌缺血机理与诊疗技术重点实验室基金(KF201015)

摘　　要：目的探讨肌肽(carnosine)对高糖诱导的大鼠心肌成纤维细胞(cardiac fibroblasts,CFs)增殖的影响及作用机制。方法以CFs为研究对象,采用WST-1法观察肌肽抑制高糖诱导CFs的增殖;乳酸脱氢酶(LDH)法检测肌肽对细胞膜的毒性作用;Western blot法观察细胞周期蛋白P21及MAPK通路相关蛋白P38、P-p38的表达水平。结果肌肽显著抑制了高糖诱导的CFs增殖,在5～30 mmol/L范围内呈一定的浓度依赖效应,且与p38 MAPK信号通路相关。结论肌肽可通过P38MAPK信号通路抑制高糖诱导的CFs增殖。Objective To study the effect of camosine on cell proliferation in rat cardiac fibro blasts （CFs） induced by high glucose and the possible mechanism. Methods CFs prolifera tion induced by high glucose was observed by WST1 assay. The eytotoxicity was detected by lactate dehydrogenase （LDH） assay. In addition, Western blot method was used to observe the expression level of cell cycle protein P21, P38 and Pp38. Results Camosine significantly in hibited proliferation of CFs induced by high glucose in concentrationdependant way within 5 - 30 mmol/L. The inhibition of eamosine was closely related to MAPK pathways. Conclusion Carnosine can inhibit proliferation of CFs by high glucose. The mechanism on eamosine＇ s inhi bition could be related to MAPK pathways.

关 键 词：肌肽 高糖 心肌成纤维细胞 细胞增殖 MAPK信号通路 糖尿病心肌病 

分 类 号：R587.1[医药卫生—内分泌]