创伤性脑损伤对骨折愈合影响动物模型的建立与评估  

Establishment and evaluation of animal model for studying the effect of traumatic brain injury on bone fracture healing

作　　者：陈旭红[1] 白露[1] 顾航宇[1] 陈博[1] 黎庆钿[1] 安帅[1] 王志永[1] 邓玖旭[1] 寇玉辉[1] 张培训[1] 殷晓峰[1] 韩娜[1] 姜保国[1] 

机构地区：[1]北京大学人民医院创伤骨科,北京大学交通医学中心,北京100044

出　　处：《北京大学学报（医学版）》2012年第6期831-837,共7页Journal of Peking University:Health Sciences

基　　金："十一五"国家科技支撑计划项目(2007BAI04B06);北京市科技新星计划(A-2008-10);国家自然科学基金(30973066;31171150;31271284;81171146;30971526;30801169);教育部新世纪优秀人才基金(BMU 20110270);高等学校博士学科点专项科研基金(20090001120096);北京市科技支撑;市政府重点工作项目(Z101107052210001);北京大学人民医院研究与发展基金(RDB2011-22)资助~~

摘　　要：目的:建立脑损伤对骨折愈合影响的动物模型,并进行稳定性评估。方法:80只成年雄性SD大鼠随机分为骨折合并脑损伤组(A组)和单纯骨折组(B组),再按5个不同时间点(6 h、1周、2周、1个月和2个月)进行取材评估,每次取8只,损伤后观察各组的组织学、神经行为学和影像学等变化。结果:A组脑损伤后大鼠90%出现偏瘫,死亡率为10%(4/40)。脑损伤严重程度评分(neurological severity score,NSS)显示造成中度脑损伤,2周后脑损伤虽有所恢复,但NSS评分值趋于稳定。光镜下脑创伤区血脑屏障破坏,神经元广泛变性坏死。伤后1周、2周、1个月和2个月A组和B组骨痂体积测量结果分别为60.03±28.05和32.80±11.04、78.54±15.16和51.36±23.02、93.01±10.65和72.38±20.38、115.26±40.00和60.30±13.34,单位为mm3,A组和B组在2周(P=0.036)、1个月(P=0.006)和2个月(P=0.010)时骨痂体积差异均具有统计学意义;在1周时两组差异无统计学意义(P=0.065)。结论:实验制作的动物模型定量准确,确实可信,稳定性及可重复性好,是研究骨折与颅脑损伤相关性的有效平台。Objective：To establish a stable animal model for studying the effect of traumatic brain injury on bone fracture healing.Methods：Eighty adult male Sprague-Dawley rats were randomly divided into fracture combined brain injury group（A） and simple fracture group（B）.Animals of the two groups were killed 6 hours,1 week,2 weeks,1 month and 2 months after trauma,respectively.Their brain histopathology changes were observed and neurological severity scores（NSS,0 through 25 from no injury to severe injury） determined to measure the brain injury after head trauma,and fracture-healing was assessed by measuring callus volume and X ray examination at the scheduled time points after trauma.The callus volumes were compared between the groups using independent-samples t test 1 week,2 weeks,1 month and 2 months after trauma respectively.A value of P0.05 was considered statistically significant.Results：Ninety percent of the rats of group A presented with hemiplegia and the mortality rate was 10%（4/40）.The survived rats developed decorticated flexion deformity of the forelimbs,with behavioral depression,and lost some reflexes and muscle tone.The NSS were 10.83±1.94,9.33±0.82,8.17±1.17,7.83±0.75 and 8.07±0.82 with 6 hours,1 week,2 weeks,1 month and 2 months after trauma,respectively.It showed that the animals received moderate head injury,which tended to be stable from 2 weeks after trauma.Brain pathology showed that blood brain barrier was destroyed,and neurons were degenerative and necrotic at and around the trauma sites.The callus volumes（unit：mm3） of the two groups 1 week,2 weeks,1 month and 2 months after trauma were 60.03±28.05 and 32.80±11.04,78.54±15.16 and 51.36±23.02,93.01±10.65 and 72.38±20.38,115.26±40.00 and 60.30±13.34,respectively.The callus volumes of the two groups 2 weeks,1 month and 2 months after trauma were statistically and significantly different（P values were 0.036,0.006 and 0.01 respectively）,and there was no difference 1 week after trauma（P=0.065）.Conclusion�

关 键 词：骨折愈合 脑损伤 模型 动物 

分 类 号：R683[医药卫生—骨科学]