比格犬严重多发伤动物模型的研制  

Establishment of an animal model of severe multiple trauma in Beagle dogs

作　　者：李庆新[1] 郑军 张世范[1] 孟辉[1] 杨俊峰 高炜[1] 刘传兰[1] 

机构地区：[1]兰州军区兰州总医院胸外科,甘肃兰州730050 [2]广州军区广州疗养院

出　　处：《西北国防医学杂志》2012年第6期601-604,共4页Medical Journal of National Defending Forces in Northwest China

基　　金：甘肃卫生行业科研计划项目(GSWST09-19);甘肃省科技支撑计划资助项目(1104FKCA106;1104FKCA069)

摘　　要：目的:研制严重多发伤动物模型,探讨动物模型的稳定性及其救生效应。方法:选健康比格犬31只,随机分组,制备严重多发伤、出血性休克模型和严重多发伤、胸腹联合伤、上呼吸道梗阻窒息模型,探讨模型指标在不同分组间的敏感度及特异度。结果:2种动物模型成型率均可满足不同实验要求,用本装置行自体输血+液体复苏,动物全部存活,单一液体复苏半数死亡;窒息组总病死率为23.1%,窒息≤6 min动物全部存活,≥9 min成活率为60%。结论:严重多发伤、上气道梗阻窒息模型、出血性休克模型符合现场临床实际,有较高的成型率和稳定性,基本涵盖了重危伤情多个层面,半数致死率和预期概率接近,为研究现场救护技术、救护装备、评价救护效能提供了依据。Objective：To establish an animal model of severe multiple trauma,and to explore its stability and rescuing effect.Methods： Thirty-one healthy Beagle dogs were randomly divided into two groups.One was the group of severe multiple trauma,haemorrhegic shock model and another was the group of severe multiple trauma,thoraco-abdominal combined trauma,upper respiratory tract obstruction asphyxia model.Sensibility and specificity between the two groups were compared.Results： Shaping rates of two groups could meet with all the requirement of different experiments.All the animals survived when autologous blood transfusion plus fluid resuscitation were taken by our patented equipment.Half of the animals survived when single fluid resuscitation was applied.The general mortality of asphyxia was 23.1%.All the animals with asphyxia for less than 6 min survived and the survival rate of animals with asphyxia for more than 9 min was 60%.Conclusion： Severe multiple trauma,upper respiratory tract obstruction asphyxia and haemorrhegic shock model is fit for on-site clinical reality with a higher shaping rate and stability.It covers several aspects of severe dangerous trauma.Half lethality is approximated to the expected probability.It provides evidence for the research of on-site rescuing skill,rescuing equipment and for the evaluation of rescuing efficacy.

关 键 词：创伤急救 上呼吸道窒息 出血休克 动物模型 比格犬 

分 类 号：R641[医药卫生—外科学]