低氧诱导因子1a在大鼠下肢急性缺血中的表达  

Expression of HIF-Ia in acute limb ischemia of rats

作　　者：李晰[1] 辛世杰[1] 杨栋[1] 丁奎[1] 汪颖厚[1] 曹辉[1] 张健[1] 段志泉[1] 

机构地区：[1]中国医科大学附属第一医院血管甲状腺外科,沈阳110001

出　　处：《中华医学杂志》2012年第45期3232-3235,共4页National Medical Journal of China

基　　金：国家自然科学基金（81170295）

摘　　要：目的了解低氧诱导因子1a（HIF-1a）在急性大鼠下肢缺血肌组织中表达的变化规律。方法以SD成年大鼠建立下肢缺血模型，根据阻断左骼总动脉的时间分别设假手术组（0h组）、4h组、8h组、12h组和24h组，切取下肢腓肠肌，通过免疫绀纵化学、免疫印迹（Western印迹）及实时定量逆转录酶聚合酶链反应（RT-PCR）检测缺血腓肠肌中HIF-1a的表达。结果HIF-1a在大鼠正常腓肠肌中呈低水平表达，而在各缺血组中的表达均明显高于假手术组（P〈0．05），并且在缺血早中期（4h和8h），其表达达到高峰，随着缺血时间的延长，HIF-1a表达旱下降趋势。结论HIF-1a在大鼠急性下肢缺血过程中存在特征性的变化趋势，推测HIF-1a可能是急性缺血过程中一种重要的适应性调节。Objective To explore the expression of hypoxia-inducible factor 1 alpha （HIF-Ia） in rat lower limb skeletal muscle with acute ischemia. Methods Acute lower limb isehemia was established in 25 male or female SD rats of 200 - 250 g by ligating left iliac artery with microsurgical techniques. Gastrocnemius of lower limb was harvest at the timepoints of sham （0h） , 4, 8, 12 and 24 h respectively. lmmunohistochemistry, Western blot and real-time polymerase chain reaction （PCR） were used to detect the expression of HIF-la in lower limb gastrocnemius. Results There was a low expression of HIF-Ia in normal gastrocnemius, but it was highly expressed in lower limb ischemia. With the elongation of isehemia time, its expression increased （ P 〈 0. 05） , peaked at 4 h and 8 h （ P 〈 0. 05） and then declined gradually. Conclusion With a characteristic trend during acute limb ischemia, HIF-Ia may play an important role in the adaptive response to acute limb ischemia.

关 键 词：缺氧诱导因子1 外周血管疾病 肌 骨骼 

分 类 号：R543[医药卫生—心血管疾病]