AVP免疫阳性反应在无蹼壁虎全脑的分布  

Distribution of AVP Immunoreactivity in the Whole Brain of Gekko Swinhonis

作　　者：赵劲松[1] 王智超[2] 汪安云[1] 鲁亚平[3] 

机构地区：[1]皖南医学院生物化学教研室,芜湖241000 [2]塔里木大学动物科学学院,新疆阿拉尔市843300 [3]安徽师范大学生命科学学院,芜湖241000

出　　处：《中国组织化学与细胞化学杂志》2012年第6期556-562,共7页Chinese Journal of Histochemistry and Cytochemistry

基　　金：安徽省高等学校省级优秀人才基金项目(2012SQRL121);皖南医学院中青年科研基金项目(WK201115)

摘　　要：目的为研究精氨酸加压素(Arginine Vasopressin)免疫阳性反应在无蹼壁虎全脑的分布。方法我们采用苏木素染色和免疫组织化学ABC法。结果发现AVP免疫阳性细胞在无蹼壁虎脑部的鲁卡斜角带核(nucleus of the di-agonal band of Broca)、视上核(supraoptic nucleus)、室旁核(paraventricular nucleus)分布较多,在室周视前区(periventricularpreoptic area)、终纹床核(bed nucleus of the stria terminalis)、下丘脑外侧区(area lateralis hypothalami)、前脑外侧束(lateralforebrain bundle)、室周核(periventricular nucleus)分布较少;AVP免疫阳性纤维除在嗅球、嗅束、大脑皮层、视叶、小脑皮层未见外,其它脑区均可见或多或少的阳性纤维;AVP阳性纤维在全脑投射广泛,且均与哺乳动物相似;AVP免疫阳性细胞数在PVN的6个部位呈中间阳性细胞数较多,两侧阳性细胞数较少(F=58.02,P<0.001)。结论上述结果显示AVP免疫阳性反应在无蹼壁虎与哺乳动物脑区间存在相似性,提示AVP在无蹼壁虎脑区执行着类似于哺乳动物的生理功能。Objective To study the distribution of AVP immunoreactivity in the brain of Gekko Swinhonis. Methods We used the hematoxylin staining and ABC method of immunohistochemistry. Results AVP immunoreaetive ceils were more densely distributed in the nucleus of the diagonal band of Broca, supraoptic nucleus and paraventricular nucleus, but less in the periventricular preoptic area, bed nucleus of the stria terminalis, area lateralis hypothalami, lateral forebrain bundle and periventricular nucleus. AVP immunoreactive nerve fibers were more or less seen in the whole brain except the olfactory bulb, olfactory tracts, cerebral cortex, cerebellar cortex and optic lobe, AVP positive fibers showed wide cerebral projection similar to that of mammalians. AVP imrnunoreactive cells were more in the middle but less on both sides in the six positions of PVN （F= 58.02, P〈0. 001）. Conclusion AVP immunoreactivity is similar between Gekko Swinhonis and mammalians, which suggests that AVP performs similar physiological functions in the brain of Gekko Swinhonis to those of mammalians.

关 键 词：无蹼壁虎 室旁核 精氨酸加压素 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]