金胺O荧光染色在石蜡组织切片麻风病诊断中的应用  

Application of auramine O fluorenscent staining to perraffin sections for the diagnosis of leprosy

作　　者：齐兴峰[1] 谢飞来[1] 朱育连[1] 郑智勇[1] 

机构地区：[1]南京军区福州总医院病理科,福建350025

出　　处：《中国组织化学与细胞化学杂志》2012年第6期595-597,共3页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：目的为了验证金胺O荧光染色法应用于石蜡组织切片麻风杆菌检测的可行性。方法用金胺O荧光法对6例确诊为麻风病的病理组织切片进行染色,并与抗酸染色结果进行对比。结果荧光染色法6例结果均为阳性,在暗背景下麻风杆菌显示明亮淡绿色荧光;在菌量较少荧光染色片中寻找单根麻风杆菌,较抗酸染色片更为容易。结论金胺O荧光染色法可用于石蜡组织切片麻风病的诊断,麻风杆菌单根散在时比抗酸染色法有一定优势。Objective To verify the feasibility of detecting M. leprae in paraffin tissue sections by au- ramine O fluorescent staining. Methods The pathological sections of 6 leprosy cases were stained by aura- mine O fluorescence, and the results were compared with those stained by acid-fast staining. Results The M. leprae were positively stained by auramine O fluorescence in all of the cases, and showed bright green fluorescence in the dark background. Compared with acid-fast staining, auramine O fluorescence was bet- ter to show single scattered M. leprae in sections with only a few bacteria. Conclusion Auramine O fluores- cent staining can be used to diagnose leprosy,as it is much more sensitive in detecting single scattered M. leprae than acid-fast staining.

关 键 词：麻风病 组织切片 金胺O荧光染色 抗酸染色 

分 类 号：R466[医药卫生—临床医学]