过敏性支气管哮喘外周血中白细胞介素17阳性调节性T细胞的表达水平变化及临床意义  

Expression of interleukin-17^+ regulatory T cells in peripheral blood of allergic asthma and its clinical significance

作　　者：王林林[1] 时国朝[1] 万欢英[1] 汤葳[1] 余荣环[2] 朱海星[1] 侯晓霞[1] 潘丽娜[1] 倪颖梦[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院呼吸科,上海200025 [2]上海市徐汇区中心医院呼吸科,上海200031

出　　处：《内科理论与实践》2012年第6期425-428,共4页Journal of Internal Medicine Concepts & Practice

基　　金：上海自然科学基金基础重点研究项目(项目编号:08JC1415200)

摘　　要：目的:探讨调节性T细胞(Treg细胞)表达白细胞介素17(IL-17)的特征及临床意义。方法:纳入对屋尘螨过敏的哮喘患者27例作为研究对象,健康志愿者25名作为对照组。采用流式细胞术检测外周血IL-17+Treg/Treg的百分比,及其与第1秒用力呼气容积占预计值百分比(FEV1%)。结果:间歇-轻度哮喘组(n=16)、中重度哮喘组(n=11)、正常对照组(n=25)IL-17+Treg/Treg百分比分别为(7.51±2.90)%、(19.13±9.80)%、(45.30±2.23)%,间歇-轻度哮喘组与正常对照组无统计学差异(P=0.054),中重度哮喘组与正常对照组及间歇-轻度哮喘组之间有明显统计学差异(分别P<0.001、P<0.01)。IL-17+Treg/Treg百分比与FEV1%呈显著负相关(r=-0.465,P<0.01)。结论:过敏性哮喘患者外周血中Treg细胞可分泌IL-17,IL-17+Treg细胞可能为Treg细胞向Th17细胞转化的中间态,IL-17+Treg细胞可能在炎症反应过程中起到重要作用,从而影响了哮喘患者的病情。Objective To study the characteristics and significance of expression of interleukin 17 （IL-17） by regulatory T cells （Treg）. Methods Twenty seven patients with house dust mites allergic asthma and 25 healthy volunteers served as control were enrolled. IL-17Treg/Treg ratio in peripheral blood was determined by flow cytometry, and forced expiratory volume in one second （FEVI） as the percentage of predicted value （FEVI% pred） was measured. Results The IL-17CFreg/Treg ratio of intermittent-mild asthma （n=16）, moderate-severe asthma （n=l 1） and control group （n=25） were （7.51±2.90）%, （19.13±9.8）% and （5.30±2.23）%, respectively. IL-17CFreg/Treg ratio in patients with intermittent-mild asthma and moderate-severe asthma was higher than that in healthy controls [（7.51±2.90）% vs （5.30±2.33）%, P=-0.054; （19.13±9.80）% vs （5.30±2.33）%, P〈0.001], and there was also a statistically significant difference between intermittent-mild asthma and moderate-severe asthma [（7.51±2.90）% vs （19.13±9.80）%, P〈0.01]. The IL-17Treg/Treg ratio was negatively correlated with FEV pred （r=-0.465, P〈0.01）. Conclusions IL-17reg/Treg ratio in peripheral blood was elevated in pa- tients with allergic asthma, and might play an important role in the course of inflammatory response, thereby influencing the severity of asthma.

关 键 词：过敏性哮喘 调节性T细胞 白细胞介素17 第1秒用力呼气容积占预计值百分比 

分 类 号：R562.25[医药卫生—呼吸系统]