血管紧张素Ⅱ对血管内皮细胞内[Ca^(2+)]i和线粒体膜电位的影响及辛伐他汀的保护作用  

Effects of angiotensin Ⅱ on [Ca2+]i and mitochondria membrane potential in vascular endothelial cell and protection of simvastatin

作　　者：李鸣皋[1] 韩磊[1] 刘昕[1] 

机构地区：[1]海军总医院空潜科,北京100048

出　　处：《转化医学杂志》2012年第3期135-138,共4页Translational Medicine Journal

基　　金：海军后勤科研基金(00-3321)

摘　　要：目的观察血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)对血管内皮细胞内[Ca2+]i和线粒体膜电位的影响及辛伐他汀的保护作用。方法将血管内皮细胞分为空白对照组(仅给予细胞培养液)、单纯AngⅡ组(细胞培养液中加入AngⅡ,使其终浓度为10-7mol/L)、AngⅡ+小剂量辛伐他汀组(在单纯AngⅡ组的基础上加入辛伐他汀,使辛伐他汀的终浓度为0.2μmol/L)、AngⅡ+大剂量辛伐他汀组(在单纯AngⅡ组的基础上加入辛伐他汀,使辛伐他汀的终浓度为2μmol/L),用激光共聚焦显微镜测量各组细胞的[Ca2+]i和线粒体膜电位水平。结果①AngⅡ组的线粒体膜电位显著低于空白对照组(P<0.01);AngⅡ+辛伐他汀组的线粒体膜电位显著高于AngⅡ组(P<0.01);AngⅡ+大剂量辛伐他汀组的线粒体膜电位水平高于AngⅡ+小剂量辛伐他汀组(P<0.05)。②AngⅡ组的[Ca2+]i显著高于空白对照组(P<0.01);AngⅡ+辛伐他汀组的[Ca2+]i显著低于AngⅡ组(P<0.01);AngⅡ+大剂量辛伐他汀组的[Ca2+]i低于AngⅡ+小剂量辛伐他汀组(P<0.05)。结论AngⅡ可引起血管内皮细胞内[Ca2+]i的显著增高及线粒体膜电位的显著降低,而辛伐他汀可逆转AngⅡ的这一作用。Objective To investigate the effects of angiotensin 11 （Ang 11 ） on [ Ca2＋ ]i and mitochondria membrane potential in vascular endothelial cell （VEC） and protection of simvastatin. Methods Cultured VECs were divided into 4 groups, control group （only given cell culture medium）, Ang Ⅱ group （ Ang Ⅱ was given with the last concentration be 10-7 mol/L）, Ang Ⅱ＋ low dosage simvastatin group （ simvastatin was given with the last concentration be 0. 2 μmol/L at the base of Ang Ⅱ group, and Ang Ⅱ ＋high dosage simvastatin group （ simvastatin was given with the last concentration at 2 μmol/L on the base of Ang Ⅱ group）. Intracellular free calcium concentration and mitochondria membrane potential were measured by laser scanning confocal microscopy. Results ①Mi- tochondria membrane potential of Ang Ⅱ group was significantly lower than that of control group（ P〈 0. 01 ）. Mitochondria membrane potential of Ang Ⅱ ＋simvastatin group was significantly higher than that of Ang II group（ P〈0. 01 ）. Mitochondria membrane potential of Ang Ⅱ ＋simvastatin group was higher than that of Ang Ⅱ group （ P〈0. 05 ）. ②Intracellular free calcium concentration of Ang Ⅱ group was significantly higher than that of control group（P〈0. 01 ）. Intracellular free calcium concentration Ang Ⅱ＋ simvastatin group was significantly lower than that of AngⅡ group（ P〈0. 01 ）. Intracellular free calcium concentration in Ang Ⅱ ＋high dosage simvastatin group was lower than that of Ang Ⅱ＋low dosage group （P〈0.05）. Conclusion Ang Ⅱ induce obvious changes of intracellular free calcium concentration and mitochondria membrane potential in VEC, and simvastatin treatment can inhibit it.

关 键 词：血管紧张素Ⅱ 血管内皮细胞 线粒体膜电位 辛伐他汀 游离钙离子浓度 

分 类 号：R331.36[医药卫生—人体生理学] R972.6[医药卫生—基础医学]