氧化高密度脂蛋白对兔树突状细胞的成熟及趋化因子分泌的影响  

Study on the maturation and secretion of chemokine of rabbit dendritic cells by oxidized-high density lipoprotein

作　　者：雷霄[1] 赵文锐[1] 傅强[2] 川玲[1] 梁英魁[1] 王升[1] 郭烽[1] 李志樑[2] 

机构地区：[1]海军总医院核医学科,北京100048 [2]南方医科大学珠江医院心血管内科,广东广州510280

出　　处：《转化医学杂志》2012年第3期147-150,共4页Translational Medicine Journal

基　　金：广东省科技计划项目(2009B030801206);广东省科技计划项目(2010B031500030)

摘　　要：目的在体外观察氧化高密度脂蛋白(oxidation-high density lipoprotein,ox-HDL)诱导兔外周血源性树突状细胞(dendritic cells,DCs)成熟情况,并观察ox-HDL对DCs趋化因子巨噬细胞炎性蛋白-1α(macro-phage inflammatory protein-1 alpha,MIP-1α)和单核细胞趋化蛋白-1(macrophage chemoattractant protein-1,MCP-1)分泌的影响情况,探讨ox-HDL对DCs成熟及趋化作用的影响。方法 12只新西兰大白兔心尖穿刺采集外周血标本,分离培养树突状细胞;同时,冻存血浆,提取HDL并氧化修饰。培养的细胞随机分为磷酸盐缓冲液组、HDL组及ox-HDL组,分别加入磷酸盐缓冲液、HDL、ox-HDL干预24 h后收集细胞,检测细胞表面CD86、主要组织相容性复合物Ⅱ(major histocompatibility complexⅡ,MHCⅡ)、CD14表达。分别于12、24、48 h取细胞培养上清液测定趋化因子MIP-1α和MCP-1。结果 ox-HDL可以上调DCs表面CD86和MHCⅡ的表达,同时下调CD14的表达,细胞的形态由不成熟往成熟转变。细胞培养上清液中MCP-1随时间和ox-HDL浓度升高而逐渐升高,而MIP-1α水平受树突状细胞是否成熟影响不大。结论 ox-HDL可以促进DCs的成熟,并增加趋化因子MCP-1的分泌,导致斑块性质的不稳定,可能与斑块的免疫反应及炎症的活化相关。Objective To study on the maturation and secretion of chemokine macrophage inflammatory protein-1 alpha （ MIP-1a） and macrophage chemoattractant protein-1 （ MCP-1 ） of rab- bit dendritic cells from peripheral blood mononuclear cell by oxidized-high density lipoprotein （ox- HDL）. Methods Peripheral blood was collected by percutaneous intracardiac from New Zealand rabbits（ n = 12）. Dendrtic cells were separated from the peripheral blood and were divited into 3 groups according to different interfere factors （ phosphoric acid buffer group, HDL group, ox-HDL group）. HDL was extracted from rabbit plasm and oxidize-modified in the same time. After 24 hours of interference, DCs were collected to detect surface antigen（ CD86 ,major histocompatibility complex I1 ,CD14）. MIP-1a and MCP-1 in the supernatant were detected at 12,24 and 48 hours during cell cultures. Results Cells in ox-HDL group were pleomorphic and rough with irregular dendrites and showed an upregulation of major histocompatibility complex 11 and CD86 as well as downregulation of CD14. MCP-1 in the supernatant was upregulated by culture time and concentration of ox-HDL. MIP-1a had little effect on the maturation of DCs. Conclusion Ox-HDL induce the mature pheno- type and MCP-1 production in DCs,which may have correlation with the immune response and in- flammatory activation in arteriosclerosis plaque and lead to plaque activities.

关 键 词：氧化高密度脂蛋白 树突状细胞 趋化因子 动脉粥样硬化 斑块 

分 类 号：R392.12[医药卫生—免疫学] R543.5[医药卫生—基础医学]