结核分支杆菌重组蛋白rps12的结构与功能预测  

Bioinformatics Analysis of the Structure and Fuction in Mycobacterium Tuberculosis Recombinant Protein rps12

作　　者：丁淑琴[1] 杨园园[2] 杨凤琴[1] 朱佳佳[1] 

机构地区：[1]宁夏医科大学,银川750004 [2]宁夏银川市第一人民医院,银川750001

出　　处：《宁夏医科大学学报》2012年第11期1130-1132,F0003,共4页Journal of Ningxia Medical University

基　　金：宁夏自然基金项目(NZ1196);宁夏医科大学校级项目基金(XM200825;XM201012)

摘　　要：目的应用生物信息学分析软件预测结核分支杆菌重组抗原rps12的结构与功能。方法应用NCBI、Expasy等在线生物信息学网站及DNAstar、Rasmol等软件包分析预测结核分支杆菌重组蛋白rps12的二级结构、三级结构;预测主要抗原表位等。结果经预测,该蛋白分子质量约为13849.2KDa,理论等电点为11.37,有6个抗原表位,其结构域位于1-34,35-124。结论生物信息学技术在结核分支杆菌rps12重组蛋白研究中有一定的理论和参考价值。Objective To predict the structure and function of recombinant antigen rps12 of Mycobacterium tuberculosis using bioinformatics method.Methods Multi-sequence homological alignment,secondary structure and tertiary structure,antigenic epitope analysis were performed by online analysis at bioinformatics websites such as NCBI（http：//www.nibi.nlm.nih.gov/）and Expasy（http：//cn.expasy.org/）,and employing software packages such as DNAstar and Rasmol.Results Analysis of the predicted protein showed that the molecular mass was 13849.2KDa,PI was 11.37,function sites were 1-34,35-124 and six antigen pitopes were found.Conclusion Bioinformatic prediction is an effective method to find and analyze the rps12 gene of mycobacterium tuberculosis recombinant protein.

关 键 词：结核分支杆菌 rps12 生物信息学 结构 功能 

分 类 号：R440[医药卫生—诊断学]