三七总甙对糖尿病皮肤溃疡大鼠肿瘤坏死因子-α水平的影响  

Influence of panax notoginsenoside on tumor necrosis factor-α in rats with diabetic skin ulceration

作　　者：于鑫[1,2,3] 董化江[1,2,3] 单娜娜[4] 徐海环[1] 赵明亮[2,3] 杨徳慧 李伯森[2,3] 单云官[2,3] 

机构地区：[1]武警后勤学院附属医院中心ICU,天津300162 [2]武警后勤学院解剖学教研室 [3]民政部101研究所遗体防腐整容重点实验室,天津300162 [4]武警后勤学院教育技术中心,天津300162

出　　处：《新乡医学院学报》2012年第12期893-895,共3页Journal of Xinxiang Medical University

基　　金：国家十一五科技支撑计划课题(编号:2007BAK38B05)

摘　　要：目的探讨三七总甙对糖尿病皮肤溃疡(DSU)大鼠肿瘤坏死因子-α(TNF-α)的影响。方法 DSU模型制作成功的Wistar大鼠60只随机分为对照组(生理盐水组)和三七总甙高剂量组(200 mg.kg-1.d-1)、中剂量组(100 mg.kg-1.d-1)、低剂量组(50 mg.kg-1.d-1),灌胃30 d,右心房取血,放射免疫法检测血清TNF-α水平,反转录聚合酶链式反应、蛋白质印迹法检测溃疡组织中TNF-αmRNA及蛋白表达。结果与对照组比较,三七总甙高、中、低剂量组血清TNF-α水平随三七总甙剂量的升高而降低(P<0.05),低剂量组高于中剂量组(P<0.05),中剂量组高于高剂量组(P<0.05)。与对照组比较,三七总甙高、中、低剂量组溃疡组织TNF-αmNRA及蛋白表达均下调,低剂量组表达高于中、高剂量组(P<0.05),中剂量组高于高剂量组(P<0.05),差异均有统计学意义。结论三七总甙可降低DSU大鼠TNF-α的表达,在一定剂量范围内可有效控制DSU炎症进展。Objective To investigate the influence of panax notoginsenoside（PNS） on tumor necrosis factor-α（TNF-α） in rats with diabetic skin ulceration（DSU）.Methods Sixty Wistar rats were used to build DSU model,then were randomly divided into control group,high-dose PNS（200 mg·kg-1·d-1）group（PNS-H group）,middle-dose PNS（100 mg·kg-1·d-1） group（PNS-M group） and low-dose PNS（50 mg·kg-1·d-1） group（PNS-L group）.The rats were fed with PNS by intragastric administration for 30 days.The level of TNF-α in serum was detected by radioimmunoassay,the expressions of TNF-α mRNA and protein in ulcer tissue were detected by reverse transcription-polymerase chain reaction and Western blotting methods.Results From the PNS-H group to PNS-L group,the level of serum TNF-α decreased gradually,which in PNS-H group decreased more than in PNS-M group（P0.05） and in PNS-M group decreased more than in PNS-L group（P0.05）.Compared with control group,the expressions of ulcer tissue TNF-α mRNA and protein in PNS-H,PNS-M and PNS-L group decreased,the expressions in PNS-L group were significantly higher than those in PNS-H and PNS-M group（P0.05）,and the expressions in PNS-M group were higher than that in PNS-H group（P0.05）.Conclusion PNS can decrease the expression of TNF-α in rats with DSU,and effectively control the progression of inflammation.

关 键 词：三七总甙 大鼠 糖尿病皮肤溃疡 肿瘤坏死因子-Α 

分 类 号：R587.2[医药卫生—内分泌]