量子点标记荧光免疫分析测定雌三醇  

Fluorescence immunoassay for determination of estriol using quantum dots as probe

作　　者：张霞[1] 杜凌云[1] 王术皓[1] 

机构地区：[1]聊城大学化学化工学院,山东聊城252059

出　　处：《分子科学学报》2012年第6期453-456,共4页Journal of Molecular Science

基　　金：国家自然科学基金资助项目(20877037)

摘　　要：采用包被雌三醇完全抗原竞争免疫分析模式,以量子点标记羊抗兔抗体为荧光探针,建立了一种测定雌三醇的量子点标记的荧光免疫分析新方法.方法测定雌三醇的线性范围为0.01～10 000μg/L;检出限为0.007 5μg/L.此法用于尿液样品的分析,并利用高效液相色谱法进行对照,测定结果一致。A sensitive and selectivity fluorescence immunoassay（FIA） was established for the determination of estriol,based on goat anti-rabbit IgG conjugated by quantum dots（QDs-IgG） as probe in this system.Estriol assay was carried out by measuring the fluorescence of QDs-IgG at 604 nm,the linear relationship between values of fluorescence intensity and logarithm of mass concentration（lgc） of estriol was obtained in the range of 0.01~10 000 μg/L with detection limit of 0.007 5 μg/L.The proposed method was applied to the analysis of samples of urine,and the results were similar to those obtained from a high performance liquid chromatography（HPLC） method,while this method is simple,rapid,sensitive,and accurate.

关 键 词：雌三醇 荧光免疫分析 量子点 

分 类 号：O641[理学—物理化学]