山茱萸总皂苷对白血病细胞HL-60的抑制作用  

To study total glycosides from Cormus officinalis on the inhibition of leukemia cells HL - 60

作　　者：曹喻灵[1] 王俊杰[1] 卢新华[1] 谷彬[1] 喻泽兰[1] 

机构地区：[1]湘南学院,湖南郴州423000

出　　处：《湘南学院学报（医学版）》2012年第4期7-9,共3页Journal of Xiangnan University（Medical Sciences）

基　　金：湖南省教育厅科研计划项目(12C0904);湘南学院药理学湖南省重点建设学科;湖南省心脑血管天然药物研究重点实验室资助

摘　　要：目的从山茱萸果肉中提取山茱萸总皂苷(total glycosides from Cormus officinalis,TGCO),观察TGCO对白血病细胞HL-60的抑制作用。方法采用乙醚脱脂乙醇回流提取法,提取山茱萸中活性成分总皂苷,用紫外分光光度计检测山茱萸提取物中总皂苷含量。以白血病细胞HL-60为实验对象,用MTT法检测TGCO作用于白血病HL-60细胞后的抑瘤作用;在荧光倒置显微镜下观察TGCO作用于白血病HL-60细胞后的生长变化。结果经紫外检测TGCO提取物OD值为0.401,皂苷类成分浓度为0.0257 g/ml,含量为0.257 g/ml。MTT法检测TGCO在5～100μg/ml浓度范围内具有抑制白血病HL-60肿瘤细胞作用,荧光倒置显微镜下观察TGCO在25～50μg/ml浓度范围内能明显改变白血病细胞HL-60的生物学特性。结论 TGCO对HL-60癌细胞有明显的抑制作用。Objiect To investigate the inhibition of TGCO（ total glycosides from Cormus officinalis） that extract from Comus officinalis pulp to human acute myloid leukemia cell line HL - 60. Methods Extract TGCO by using 75 % ethanol reflux, UV was used to determine the content of total glycosides. Leukemia cell line HL - 60 was used as in vitro model. HL - 60 was treated with TGCO at different concentrations, cell growth was analyzed with inverted fluorescence microscope and MTT assay. Results TGCO OD is 0.401, which detected by UV. The concentration of glycosiders is 0.0257 g/ml, the content is 0. 257 g/ml. 5 - 50pg/rnl of TGCO could inhibit leukemia cell line HL - 60. Conclusion TGCO significantly inhibeted leukemia cell line HL - 60.

关 键 词：山茱萸总皂苷 提取 白血病细胞HL-60 抑制作用 

分 类 号：R285.5[医药卫生—中药学]