抗铅微生物的筛选及EDDS螯合诱导黑麦草修复铅污染土壤的效应初探  

Screening of Lead Resistant Microorganism and Preliminary Study on the Effect of Repairing Lead Polluted Soil by EDDS Chelating Induced Ryegrass

作　　者：曹铁华[1] 牟忠生[1] 王淑萍 闫海洋[1] 梁烜赫[1] 范作伟[1] 金荣德[1] 

机构地区：[1]吉林省农业科学院,长春130033 [2]龙井市农业技术推广中心,吉林龙井133400

出　　处：《吉林农业科学》2012年第6期32-34,50,共4页Journal of　Jilin Agricultural Sciences

基　　金：吉林省科技厅国际合作项目(20100722)

摘　　要：从污染的土壤中筛选出两株较高抗铅细菌(CJ1,CJ2)和两株真菌(ZJ1,ZJ2),经过菌株的液体培养对铅的除去率达到了60.6%、51.3%、78.7%、47.2%。其中选择两种去除率高的细菌CJ1和真菌ZJ1,通过黑麦草的盆栽试验,在其内添加不同种类的微生物、EDDS(乙二胺二琥珀酸)及微生物与螯合剂的复合调控,分析了黑麦草吸收铅能力。结果发现,EDDS和真菌的复合处理下黑麦草根系和叶片中的铅含量分别比对照增加了7.7倍和10.68倍。说明复合处理对铅在黑麦草体内的富集和传输上有显著的诱导作用。Two strains of bacteria （CJ1, CJ2） and fungi （ZJ1, ZJ2） were isolated from the lead-contami- nated soil to investigate their efficacy in decrease lead-contamination. After liquid cultivation in the medium containing high levels of lead, the lead removal rate of each strain were reached at 60.6%, 51.3%, 78.7% and 47.2%, respectively. Then, two strains （CJ1 and fungi ZJ1） which showed the highest removal rate were se- lected for further study. Two strains, ryegrass, EDDS （ethylenediamine succinic acid） and chelating agent was combined at different composition to study their efficacy in lead uptake. It was found that combining EDDS with ZJ1 showed the best efficacy and the lead content in the roots and leaves of ryegrass were increased by 7.7 times and 10.68 times compared to control, respectively. In a conclusion, the application of ryegrass with combined treatment would stimulate lead uptake by absorption and transmission.

关 键 词：微生物筛选 螯合剂 植物 去除率 吸收量 

分 类 号：S154.3[农业科学—土壤学]