苦参碱对人食管癌细胞株Eca-109增殖和凋亡的影响  

Investigate the Effects of Matrine on the Proliferation and Apoptosis of Human Esophageal Carcinoma Eca-109 Cell Line

作　　者：杜好信[1] 曹云鹏[2] 耿国军[1] 姚成才[1] 陈隽鹏[1] 张义[1] 陈端扬[1] 姜杰[1] 

机构地区：[1]福建厦门市中医院胸外科,厦门361009 [2]福建厦门大学医学院,厦门361005

出　　处：《光明中医》2012年第6期1096-1101,共6页GUANGMING JOURNAL OF CHINESE MEDICINE

基　　金：福建厦门市卫生局科研项目(No:2007-CXB-B)

摘　　要：目的探讨中药提取物苦参碱单体(Matrine)对人食管癌细胞株Eca-109的诱导凋亡和抑制增殖作用。方法体外培养人食管癌细胞株(Eca-109),分别给以不同浓度的苦参碱对体外培养的Eca-109细胞株进行干预。以MTT比色法、Hoechst33342染色法以及流式细胞术测定苦参碱对Eca-109细胞株的诱导凋亡及抑制增殖的作用。结果 MTT实验显示苦参碱可以明显抑制Eca-109细胞株的增殖,流式细胞术检测细胞周期显示G2期细胞明显增多,S期细胞显著减少。结论苦参碱能明显抑制Eca-109细胞株的增殖,促进其凋亡,其作用机制可能与细胞周期阻滞于G2期有关。Objective To investigate the effects of matrine on the proliferation and apoptosis of human esophageal carcinoma Eca-109 cell line.Methods Different concentrations of matrine were used in the culture of esophageal carcinoma cells.Proliferation,apoptosis,and cell cycle arrest of esophagus carcinoma Eca-109 cells line were observed by MTT assay,Hoechst33342 staining,and flow cytometry quantitative analysis.Results showes that matrine significantly induced apoptosis of the Eca-109 cell line;The cell cycles measurement by flow cytometry quantitative analysis showed an increase in G2 phase cells,and S phase cells were significantly reduced.Conclusions Matrine can inhibit proliferation and promote apoptosis of the Eca-109 cell line,and the mechanism may be correlated to the cell cycle arrest at G2 phase.

关 键 词：苦参碱 食管癌细胞 增殖 凋亡 细胞周期 

分 类 号：R285.5[医药卫生—中药学] R735.1[医药卫生—中医学]