高速逆流色谱法分离野马追乙酸乙酯萃取物中三种倍半萜烯内酯  

Isolation and Purification of three Sesquiterpenoid Lactones from Eupatorium Lindleyanum DC Ethyl Acetate Fraction by High-speed Counter-current Chromatography

作　　者：鄢贵龙[1,2,3] 纪丽莲[2] 罗玉明[2] 胡永红[3] 

机构地区：[1]淮阴师范学院江苏省生物质能与酶技术重点实验室,江苏淮安223300 [2]淮阴师范学院江苏省环洪泽湖生态农业生物技术重点实验室,江苏淮安223300 [3]南京工业大学生物与制药工程学院,江苏南京210009

出　　处：《淮阴师范学院学报（自然科学版）》2012年第3期261-265,共5页Journal of Huaiyin Teachers College;Natural Science Edition

基　　金：江苏省研究生科研创新计划项目(CX10B-182Z);江苏省生物质能与酶技术重点实验室课题项目(JSBEET1222)

摘　　要：建立了应用高速逆流色谱(HSCCC)从野马追乙酸乙酯萃取物中分离纯化3种倍半萜烯内酯的方法.采用正己烷-乙酸乙酯-甲醇-水(1:4:2:3)作为两相溶剂体系,从900mg野马追乙酸乙酯萃取物中分离得到3β-Hydroxy-8β-[4′-hydroxytigloyloxy]-costunolide 6.5 mg,野马追内酯A28.5mg和野马追内酯B29.3 mg,其纯度分别为92.5%,91.7%和93.9%.3种化合物的结构通过ESI-MS和1H NMR进行了鉴定.A high-speed counter-current chromatography（HSCCC） method was established for the separation of three sesquiterpenoid lactones from Eupatorium lindleyanum DC. n-butanol fraction. The two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water （1：4：2：3） was selected. From 900 mg of n-butanol fraction of Eupatorium lindleyanum DC, 6.5mg of 3β-Hydroxy-8β- [4＇-hydroxytigloyloxy ]-costunolide, 28.5 mg of eupalinolide A and 29.3 mg of eupalinolide B were obtained in one-step HSCCC separation, with the purity of 92.5%, 91.7% and 93.9%, respectively, as determined by HPLC. Their structures were further identified by ESI-MS and 1H NMR.

关 键 词：野马追 高速逆流色谱 倍半萜烯内酯 分离 

分 类 号：R284.2[医药卫生—中药学]