胆汁泡蛋白ELISA检测法的建立与初步临床应用  

Establishment of an ELISA for biliary vesicular protein and its preliminary clinical study

作　　者：蔡端[1] 项建斌[1] 张延龄[1] 马保金[1] 王丽影[2] 

机构地区：[1]上海医科大学附属华山医院外科教研室,200040 [2]上海医科大学基础医学院生化教研室

出　　处：《上海医学》2000年第6期341-344,共4页Shanghai Medical Journal

基　　金：生部基金! ( 98 2 12 4)

摘　　要：目的　建立胆汁泡蛋白快速检测法 ,筛选有效的胆石症防治手段。方法　获取 33.5kd胆汁泡蛋白 ,通过微量抗原免疫法获得高效价抗体 ,建立ELISA标准曲线 ;并应用ELISA法测定正常人、胆石症患者胆汁和血清中泡蛋白含量 ,同时观察不同溶石防石药物如利胆冲剂、胆酸钠等对泡蛋白的影响。结果　建立了ELISA标准曲线 ,其曲线方程为Y =0 .0 35X(r=0 .99) ;正常人胆汁和血清中 33.5kd泡蛋白含量都明显较胆固醇结石患者低 (0 .0 0 7± 0 .0 0 3)mg/mlvs(0 .0 5 0± 0 .0 11)mg/ml,(0 .0 15± 0 .0 10 )mg/mlvs(0 .0 45± 0 .0 2 1)mg/ml,P <0 .0 5 ;利胆冲剂治疗 2周后 ,胆汁和血清泡蛋白含量显著降低 ,而对照组和胆酸钠治疗组未见明显变化。结论　在不同人群胆汁泡蛋白的含量差异有显著性 ;利胆冲剂等溶石防石药物能降低胆汁和血清 33.5kd泡蛋白含量 ,改变胆汁致石性 。Objectives To establish a rapid and precise detective method of 33.5 kDa vesicular protein and to screen an effective drug for cholelithiasis. Methods Specific antibody of the biliary vesicular protein was obtained by immunizing rabbits, with a view to develop an enzyme linked immunosorbent assay (ELISA) kit. The concentrations of 33.5 kDa vesicular protein in serum and bile of gallstone patients and controls were examined respectively. The effects on decreasing 33.5 kDa vesicular protein by cholagogue dry syrup and sodium cholate were also studied. Results One step ELISA provided a simple and sensitive means of measuring the biliary vesicular protein in serum and bile and its equation was Y=0.035X(r=0.99). The vesicular protein concentration in serum and bile of cholesterol gallstone group were 0.045±0.021 mg/ml and 0.050±0.010 mg/ml, respectively, significantly ( P <0.05) higher than those in the pigment stone group (0.017±0.011 mg/ml and 0.014±0.009 mg/ml, respectively) and controls ( 0.015 ±0.010 mg/ml and 0.007±0.003 mg/ml, respectively). The data showed that, when adminisered for two weeks, cholagogue dry syrup significantly decreased both biliary and serum 33.5 kDa vesicular protein in cholesterol stone patients, while sodium cholate and control groups showed no significant changes. Conclusions ELISA is a rapid and reliable method of detecting serum and biliary vesicular protein, the concentrations of 33.5 kDa vesicular protein were different in cholesterol gallstone patients and health subjects. which might be related to cholesterol nucleation process. Cholagogue dry syrup is valuable in modifying the lithogenic bile. (Shanghai Med J, 2000,23∶341 344)

关 键 词：酶联免疫法 胆汁泡蛋白 胆结石 诊断 

分 类 号：R446.61[医药卫生—诊断学]