bFGF促进内皮细胞β_1整合素表达及其意义探讨  

Effect of bFGF on expression of endothelial cell integrin β_1 subunits

作　　者：周序珑 邓漪平[2] 

机构地区：[1]暨南大学医学院病理教研室,广东广州510632 [2]中山医科大学组胚教研室,广东广州510089

出　　处：《中国病理生理杂志》2000年第6期519-521,共3页Chinese Journal of Pathophysiology

摘　　要：目的 :观察碱性成纤维细胞生长因子对血管内皮细胞 β1整合素表达的影响。方法 :采用WesternBlot法结合图像处理分析 ,检测bFGF作用前后培养血管内皮细胞 β1整合素的表达。结果 :bFGF处理组和对照组平均灰度值分别为 16 6 11± 9 86和 175 32± 5 12 ,两组免疫酶显色有显著差异。结论 :bFGF能够诱导内皮细胞膜表面β1整合素合成增加。推测bFGF通过上调内皮细胞 β1整合素表达 ,可能在促进血管生成方面有重要作用。AIM: To study the effect of basic fibroblast growth factor(bFGF) to the expression of integrin β 1 subunit of endothelial cells. METHOD: The expression of integrin β 1 subunit of endothelial cells was determined before and after bFGF treatment with Western Blot and immage analysis method.RESULTS: The mean gray value of immunostain by immage analysis method is 166.11±9.86 in the experimental group and 175.32±5.12 in the control group, suggested that bFGF may upregulate expression of integrin β 1 subunits of endothelial cell. CONCLUSION: bFGF may play an important role in angiogenesis by way of inducing the overexpression of endothelial cell integrin β 1 subunits.

关 键 词：新生血管化 内皮细胞 BFGF Β1整合素 

分 类 号：R543.02[医药卫生—心血管疾病]