快速检测Fenton反应诱导DNA损伤的电化学DNA传感器研究  被引量:6

Electrochemical DNA Sensors for Rapid Detection of DNA Damage Induced by Fenton Reaction

在线阅读下载全文

作  者:吕翠[1] 康天放[1] 鲁理平[1] 熊岳[1] 

机构地区:[1]北京工业大学环境与能源工程学院,北京100124

出  处:《分析化学》2012年第12期1822-1826,共5页Chinese Journal of Analytical Chemistry

基  金:北京市教委科技发展基金重点项目(No.KZ201110005006);北京市自然科学基金(No.8102009);北京市人才强教深化计划--创新团队(No.PH R201007105)资助

摘  要:利用带正电荷的聚二甲基二烯丙基氯化铵(PDDA)和带负电荷的小牛胸腺双链DNA(CT-dsDNA)之间的静电吸引作用,将其层层组装到玻碳电极表面,制成电化学DNA传感器,并利用电化学和原子力显微镜(AFM)方法与对DNA的组装进行了表征。以Ru(bpy)23+作为电化学催化剂,检测DNA的损伤,考察了电极在Fenton试剂中的温育时间以及Fenton试剂的浓度对DNA损伤程度的影响。实验表明,Fe2+与H2O2共存时,温育15 min即可较大程度地对DNA造成损伤,且可以检测到的Fenton试剂浓度为5.0×10"5mol/L Fe-SO4/2.0×10"4mol/L H2O2。本方法具有灵敏度高、重现性好等优点。Layer-by-layer films of poly dimethyl diallyl ammonium chloride and DNA were assembled on the surface of a glassy carbon electrode to prepare DNA electrochemical sensor.The assembling of DNA was characterized by employing electrochemical methods and atomic force microscope.We used Ru(bpy)2+3 as electrochemical catalyst to detect the oxidative damage of DNA in the membrane.The effects of incubation time and concentration of Fenton reagent on the damage of DNA were studied.The result showed that DNA could be damaged greatly when it was incubated in Fenton reagent for 15 min.The detectable concentration of Fenton reagent was 5.0×10-5 mol/L FeSO4/2.0×10-4 mol/L H2O2.The electrochemical method using Ru(bpy)2+3 as catalyst possesses high-sensitivity for detecting the damage of DNA.

关 键 词:FENTON反应 DNA损伤 层层组装 DNA电化学传感器 

分 类 号:O657.1[理学—分析化学] TP212.2[理学—化学]

 

参考文献:

正在载入数据...

 

二级参考文献:

正在载入数据...

 

耦合文献:

正在载入数据...

 

引证文献:

正在载入数据...

 

二级引证文献:

正在载入数据...

 

同被引文献:

正在载入数据...

 

相关期刊文献:

正在载入数据...

相关的主题
相关的作者对象
相关的机构对象