病原物诱导型植物启动子的研究方法  

Progress in Pathogen-inducible Promoters of Plants

作　　者：马睿[1,2] 陈华民[2] 吴茂森[2] 吴祖建[1] 何晨阳[2] 

机构地区：[1]福建农林大学植物病毒研究所福建省植物病毒学重点实验室,福州350002 [2]中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京100193

出　　处：《生物技术通报》2012年第11期32-37,共6页Biotechnology Bulletin

基　　金：国家转基因生物新品种培育重大专项(2009ZX08009-044B;2009ZX08012-016B)

摘　　要：植物启动子是控制基因转录的DNA序列之一,按其表达方法可分为组成型(或非特异性)表达和诱导型(或特异性)表达启动子两种类型。当受到病原物侵染时,植物通常通过诱导型启动子中的W-box、RAV1 AAT和WRKY等元件/位点、调控下游基因的表达,作出相应的应答反应。近年来,通过计算机预测与试验测定方法的有效结合,发掘和鉴定出了一些病原物诱导型启动子,并进行了调控功能的解析。此外,在通过转基因方法改良植物抗病性的途径中,利用病原物诱导型启动子可以实现对抗病功能相关基因表达的精准控制,避免由组成型启动子持续驱动基因表达、对植株本身带来的负效应。结合本室对水稻启动子OsBTF3-p的研究结果,重点对近年来国内外有关病原物诱导型启动子及其调控元件/位点的研究方法和应用进展作一综述。Promoter controlling the gene transcription in plant can be classified constitutive promoter（non-specific promoter）and inducible promoter（specific promoter）by the way of expression.When induced by pathogens,the W-box、RAV1AAT and WRKY elements of inducible promoter in plant can regulate the expression of downstream genes,then the plant can make the appropriate responses.Recently,many pathogen-inducible plant promoters were found and identified by computer prediction and experiment,at the same time,regulation function of promoters was analyzed.In addition,in the process of improving the resistance of plant by transgenic approaches,the choice of pathogeninducible plant promoters can achieve the accurate regulation of resistance-related genes,avoiding the negative effect in plant due to consistent expression of constitutive promoters.This paper reviews the research methods and application progresses of pathogen-inducible plant promoters and regulatory elements,combining with the results of promoter OsBTF3p.

关 键 词：诱导型启动子 调控元件 转基因途径 

分 类 号：S432[农业科学—植物病理学]