金黄色葡萄球菌耐消毒剂基因及抗生素耐药相关基因检测  

Drug-resistant gene and disinfectant-resistant gene in Staphylococcus aureus

作　　者：陈迎晓[1] 刘俊[2] 李小四[2] 林镯[1] 周铁丽[2] 陈永平[1] 

机构地区：[1]温州医学院附属第一医院感染科,浙江温州325000 [2]温州医学院附属第一医院实验检验中心,浙江温州325000

出　　处：《中国微生态学杂志》2012年第12期1103-1105,共3页Chinese Journal of Microecology

基　　金：温州市科技局项目(Y20090382)

摘　　要：目的了解温州地区临床分离的金黄色葡萄球菌(SA)的耐药特点,探讨SA中耐β-内酰胺类、氨基糖苷类、四环素类药物耐药基因及耐消毒剂基因(qacA)的存在情况。方法采用聚合酶链式反应(PCR)法对SA进行β-内酰胺酶基因、氨基糖苷类修饰酶基因、四环素类基因和耐消毒剂基因检测。结果 PCR结果显示94株SA中耐药相关基因检出率mecA 53.2%、aac(6')/aph(2″)68.1%、aph(3')-III 37.2%、tetM 53.2%和qacA 7.4%,其中59株MRSA的耐药相关基因检出率分别为mecA 83.1%、aac(6')/aph(2″)86.4%、aph(3')-III 42.4%、tetM 76.4%和qacA 8.5%。结论多数SA菌株存在耐β-内酰胺类、氨基糖苷类、四环素类等多种抗生素耐药基因,具有多重耐药特征,但尚未出现明显耐消毒剂状况。Objective To study the drug resistance genes and disinfectant-resistant genes of clinical Staphylococcus aureus isolates.Methods Common drug-resistant genes associated with β-lactamases,aminoglycosides,tetracyclines and disinfectants were detected by PCR.Results The positive rates of drug resistant genes in 94 SA strains were 53.2% for mecA,68.1% for aac（6′）/aph（2″）,37.2% for aph（3′）-III,53.2% for tetM and 7.4% for qacA.The positive rates of drug resistant genes in 59 MRSA strains were 83.1% for mecA,86.4% for aac（6′）/aph（2″）,42.4% for aph（3′）-III,76.4% for tetM and 8.5% for qacA.Conclusion There are resistance genes of β-lactams,aminoglycosides,and tetracycline in most of the SA strains,but the disinfectants-resistant genes are not prevalent.

关 键 词：金黄色葡萄球菌 耐药基因 耐消毒剂基因 

分 类 号：R378.11[医药卫生—病原生物学]