城市污水处理厂污水处理系统中四环素类药物耐药菌的分布特性研究  

Study on the distribution characteristics of tetracyclines resistant bacteria in wastewater treatment plant

作　　者：刘冲[1] 黄满红[1,2] 肖斌[1] 

机构地区：[1]东华大学环境科学与工程学院,上海201620 [2]清华大学环境学院,北京100084

出　　处：《环境污染与防治》2012年第12期5-8,共4页Environmental Pollution & Control

基　　金：国家自然科学基金资助项目(No.21007010);教育部博士点基金资助项目(No.20090075120007);中央高校基本科研基金资助项目;交通运输部科技项目(No.2010353343290);上海市科学技术委员会科研计划项目(No.09230500200);湖南省交通厅科研项目(No.200908)

摘　　要：采用微生物平板涂布法对2家城市污水处理厂污水处理各阶段的四环素类药物(四环素、金霉素、土霉素)耐药菌分布进行了测定,分析了污水处理的各阶段对四环素类药物耐药菌的去除效果。结果显示,2家污水处理厂污水处理系统中均能检出土霉素、四环素、金霉素耐药菌,数量分布均为土霉素耐药菌>四环素耐药菌>金霉素耐药菌。3种耐药菌的浓度均在加氯消毒出水中为最小(在0～103 cfu/mL),在外排污泥中最大(高于进水浓度,在105～107 cfu/mL)。四环素耐药菌数从进水的1.1×105～1.6×105 cfu/mL降为出水的0～5.5×102 cfu/mL,说明污水处理过程对四环素耐药菌有一定的去除效果,但四环素耐药菌占总菌数的百分比没有明显下降或上升,相比于进水的1.1%～3.4%,出水仍然保持在3.1%～4.3%左右,表明污水处理过程中没有选择性的富集或去除四环素耐药菌。Spread plate method was used to determinate the distribution of tetracyclines （TCs） resistant bacteria in two municipal wastewater treatment plant. The performance of different treatment process for TCs resistant bacte- ria removal was investigated. Three TCs-resistant bacteria were detected in both municipal wastewater treatment plants; they were oxytetracycline resistant bacteria, tetracycline resistant bacteria and chlortetracycline resistant bac- teria. The conlony density of three TCs-resistant bacteria followed the order of xytetracycline resistant bacteria〉tet-racycline resistant bacteria〉chlortetracycline resistant bacteria. The colony counts of TCs-resistant bacteria were smallest in chlorination effluent （about 0-10^3 cfu/mL） and highest in waste sludge （about 10^5-10^7 cfu/mL）. The wastewater treatment process had perfect efficiency for tetracycline resistant bacteria removal, the colony counts of tetracycline resistant bacteria in iniluent was decreased from 1.1×10^5～1.6×10^5 cfu/mL to final effluent of 0～5.5×10^2 cfu/mL. While, the percentage of tetracycline resistant bacteria in total TCs-resistant bacteria did not present significantly decline, it was 1.1%-3.4 % in influent water and maintained 3.1%-4.3 % in final effluent water. It could be concluded that the wastewater treatment process was not selective for enrichment or removal of tetracycline resist-ant bacteria.

关 键 词：城市污水厂 四环素类耐药菌 分布特性 

分 类 号：X703[环境科学与工程—环境工程]