甲状腺激素对新生鼠脑NOS基因表达的调节  

Regulatary Effect of Thyroid Hormone on the Expression of nNOS Gene in Neonatal Rat Brain

作　　者：陈源[1] 罗敏[1] 蔡东升[1] 丁伟[1] 陈刚[1] 宗阳[1] 

机构地区：[1]上海第二医科大学瑞金医院上海市内分泌研究所,上海200025

出　　处：《上海第二医科大学学报》2000年第3期207-210,共4页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：国家自然科学基金资助项目! (39570 347)

摘　　要：目的观察不同日龄新生鼠脑一氧化氮 (NO)含量 ,一氧化氮合酶 (NOS)活性的变化及甲状腺激素对nNOS基因表达的调节。　方法采用丙基硫氧嘧啶 (PTU)灌注孕母鼠造成甲减模型。采用NO、NOS的生化测定法以及半定量逆转录聚合酶链反应 (RT -PCR)。　结果生化测定法显示 ,不同年龄段大鼠随着年龄的增加NO含量上升 (P <0 .0 1) ,NOS的活性上升 (P <0 .0 1) ;同一年龄段仔鼠 ,甲减组较正常组NO含量下降 (P <0 .0 1) ,NOS活性下降 (P <0 .0 1) ;RT -PCR测定显示 ,幼鼠nNOSmRNA水平随着年龄的增加而上升 ;同一年龄组 ,甲减组较正常组nNOSmRNA表达下降。　结论提示甲状腺激素对nNOSmRNA表达有上调作用 ;Objective To investigate nitric oxide (NO) contents, nitric oxide synthase (NOS) activities and expression regulation of nNOS gene by thyroid hormone(TH) in neonatal rats of different ages. Methods Perinatal hypothyroidism was induced by infusing PTU solution to the dams (1% PTU 0.01 ml/g per day)beginning at 15th embryonic day. Biochemistry measurement and semi-quantitative polymerse chain reaction (RT-PCR) analysis were used. Results Biochemical measurement showed that both NO content and NOS activities rose with the ages, and they were lower in hypothyroidism rats than age matched controls (P<0.01). RT-PCR analysis showed that nNOS mRNA levels (corrected by GAPDH) also raised with the ages, and decreased significantly in hypothyroidism rats of different ages(P<0.01). Conclusion The expression of nNOS gene was up-regulated by TH.NO may take part in the course of impairment of brain development induced by TH deficiency.

关 键 词：甲状腺激素 新生鼠 脑损害 甲状腺机能减退 NO 

分 类 号：R96[医药卫生—药理学]