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机构地区:[1]广州中医药大学第二附属医院麻醉科,广东广州510120 [2]扬州大学临床医学院,江苏省苏北人民医院麻醉科,江苏扬州225001 [3]扬州大学临床医学院,江苏省苏北人民医院科教处,江苏扬州225001
出 处:《实用临床医药杂志》2012年第23期1-4,共4页Journal of Clinical Medicine in Practice
基 金:广东省中医药管理管局资助项目(2050069)
摘 要:目的观察牛珀至宝微丸对肺损伤早期纤维化大鼠转化生长因子β1(TGF-β1)的影响。方法 48只SD大鼠随机均分为4组:对照组(C组)、内毒素组(LPS组)、低剂量牛珀至宝微丸预处理组(LD组)、高剂量牛珀至宝微丸预处理组(HD组)。采用气管内联合腹腔注射内毒素法复制肺损伤早期纤维化模型。实验结束前留取标本测定肺血管通透性(EB值)及肺组织湿/干重量比(W/D);HE染色光镜下观察肺损伤程度(DAD评分);用免疫组化法和蛋白质免疫印迹法测定肺组织TGF-β1蛋白表达;Van Gieson染色法检测肺组织胶原纤维表达。结果与LPS组比较,LD组以及HD组,尤其是HD组的EB、W/D值和DAD评分明显降低(P<0.01),光镜下肺肺损伤及纤维化的程度均减轻,肺组织中TGF-β1表达明显减少(P<0.01)。结论牛珀至宝微丸通过抑制肺组织TGF-β1蛋白的激活,有助于减轻内毒素所致肺损伤早期纤维化。Objective To observe the effect of Niupo zhibao micropill on expression of trans- forming growth factor beta 1 (TGF-β1) in rats with early fibrosis of pulmonary injury. Methods 48 SD rats were randomly divided into the following 4 groups: the control group (group C) , the endotoxin group (LPS group), pretreatment group with low dosage Niupo zhibao micropill (LD group)and pre- treatment group with high dosage Niupo zhibao micropill (HD group). Model of early fibrosis of pul- monary injury was set by intratracheal and intraperitoneal injection of endotoxin. Before experiment was ended, the specimens of 4 groups were collected for assay of pulmonary vascular permeability(EB) and pulmonary tissue wet/dry proportion by weight (W/D) ; degree of pulmonary injury was observed un- der HE microscopy ( DAD score) ; Immunohistochemistry and Western blot method were used for detec- tion of TGF-β1 expression;Van Gieson staining method was used for testing of collagen fibrils expres- sion of pulmonary tissue. Results Compared with LPS group, the EB, W/D ratio and DAD score in LD group and liD group decreased significantly ( P 〈 0.01 ), degree of pulmonary injury and fibrosis relieved under microscopy, and TGF-β1 expression in pulmonary tissue reduced obviously (P 〈 0. 01 ). Conclusion Niupo zhibao micropill can suppress activation of TGF-β1 in pulmonary tissue and relieve early fibrosis of pulmonary injury induced by endotoxin.
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