RT-PCR法检测尿液标本中麻疹病毒核蛋白(N)基因的临床应用及意义  

Clinical application and significance of detection of measles virus nucleoprotein(N)gene in urines from patients with measles by RT-PCR

作　　者：王伟[1] 王海梅[1] 刘双成[1] 郑照军[1] 郑晶[1] 乔艳[2] 钟政荣[1] 

机构地区：[1]蚌埠医学院第一附属医院检验科,蚌埠233004 [2]蚌埠医学院第一附属医院感染科

出　　处：《山西医科大学学报》2012年第12期911-913,共3页Journal of Shanxi Medical University

基　　金：蚌埠医学院科研基金资助项目(BY1036)

摘　　要：目的探讨逆转录-聚合酶链反应(RT-PCR)直接从临床麻疹患者的尿液中检测麻疹病毒特异性核蛋白(Measles vi-rus nucleoprotein,MV-N)基因诊断价值。方法选取2010-2011年安徽省蚌埠市散发的15例临床确诊为麻疹的患者,采集尿液,提取RNA,用RT-PCR方法扩增麻疹病毒核蛋白的515 bp核苷酸片段。同时采集同一患者血清,进行MV-IgM抗体检测,并比较两种方法的阳性率。结果尿液RT-PCR法检测MV-N基因,15例患者中有14例扩增出阳性产物,阳性率为93.3%,高于血清MV-IgM抗体检测的阳性率60.0%(χ2=9.60,P=0.002)。结论麻疹病人尿液标本的RT-PCR检测可用于麻疹病毒的早期快速辅助诊断,特别是对于出疹初期的患者。同时对血清MV-IgM抗体阴性的病例进行此法复检,可弥补漏检。Objective To explore the clinical value of directly detecting measles virus-specific nucleoprotein gene（MV-N）from clinically diagnosed measles patients＇ urine using reverse transcription-polymerase chain reaction（RT-PCR）.Methods Urine was collected from 15 patients clinically diagnosed with measles from 2010 to 2011 in Bengbu,Anhui province.RNA was extracted from urine.RT-PCR was used to amplify 515 bp nucleotide fragment of the measles virus nucleoprotein.Serum of the same patient was collected to detect MV-IgM antibody.The positive rate was compared between two methods.Results Positive products were successfully amplified in 14 of 15 patients by RT-PCR method.The positive rate was higher by RT-PCR method than by MV-IgM detection method（93.3% vs 60.0%,χ2=9.60,P=0.002）.Conclusion RT-PCR for detecting the urine can be used as an early fast-assisted diagnosis method for measles virus,especially for patients with early rash.In the meantime,re-examination of negative MV-IgM in serum could reduce the missed diagnosis.

关 键 词：RT-PCR 麻疹病毒 核蛋白基因 

分 类 号：R446[医药卫生—诊断学]