高效液相色谱法测定可口革囊星的ACE活性  

To determine ACE activity of the Phascolosoma esulenta by HPLC

作　　者：庹康秀[1] 廖共山[1] 雷丹青[1] 

机构地区：[1]广西医科大学医学科学实验中心,广西南宁530021

出　　处：《蛇志》2012年第4期349-351,共3页Journal of Snake

摘　　要：目的利用高效液相色谱法测定可口革囊星虫酶解物中具有抑制血管紧张素转化酶(ACE)活性的活性肽。方法用HPLC测定血管紧张素转换酶抑制肽活性,在该色谱条件下,可通过测定由ACE水解马尿酰组氨酰亮氨酸后产生的马尿酸峰面积或含量得到酶解液的活性。结果酶解物在质量浓度为0.48mg/ml时对ACE的抑制率为17.62%。结论可口革囊星虫蛋白经碱性蛋白酶水解后得到的酶解物活性较高。Objective To determine the Phascolosoma esulenta hydrolysates active peptides that inhibit angioten- sin-converting enzyme （ACE） activity by HPLC. Methods Determination of angiotensin-converting enzyme in- hibitory peptide activity in the chromatographic conditions,by measuring the activity generated by ACE hydrolysis of hippurate acyl group of the aminoacyl leucine hippuric acid peak area or the content of the hydrolysates. Re- sults ACE inhibitory hydrolysates from the mass concentration 0. 48 mg/ml rate of 17.62%. Conclusion Phas- colosoma esulenta protein after hydrolysis can get hydrolysates of higher activity.

关 键 词：可口革囊星虫 高效液相色谱法 血管紧张素转化酶抑制剂 

分 类 号：S917.4[农业科学—水产科学]