转HJC-1和G6-EPSPS基因抗虫耐草甘膦水稻表达蛋白在模拟胃肠环境中的稳定性研究  

Stability of HJC-1 gene and G6-EPSPS gene protein in simulated gastric and intestinal fluid

作　　者：张力[1] 王静[1] 王晓军[1] 刘洪亮[1] 

机构地区：[1]天津市疾病预防控制中心卫生检验所,天津300011

出　　处：《环境与健康杂志》2012年第12期1074-1076,共3页Journal of Environment and Health

基　　金：国家"十一五"转基因重大专项(2008ZX08011-005)

摘　　要：目的研究HJC-1和G6-EPSPS基因表达的蛋白分别在模拟胃液和模拟肠液中的消化稳定性。方法采用美国1995年药典提供的模拟胃液和模拟肠液配方,在体外建立模拟胃肠环境消化体系,测定HJC-1和G6-EPSPS基因表达的蛋白质在胃肠环境中的稳定性。蛋白质在模拟胃、肠液中的浓度分别为5.0和2.0 mg/ml。在蛋白质与模拟胃、肠液反应后的0、15、30 s,1、2、5、10、20、30和60 min准确取样,根据SDS-PAGE凝胶电泳结果,判断蛋白质在模拟胃、肠液环境中的稳定性。结果 HJC-1基因表达的蛋白质在模拟胃液和模拟肠液中均在15 s内全部降解;G6-EPSPS基因表达的蛋白质在模拟胃液中30 s内全部降解,在模拟肠液中60 min内不能完全降解。结论 HJC-1基因表达的蛋白质在模拟人体胃肠环境中不稳定,易被降解。G6-EPSPS基因表达的蛋白质在模拟人体胃环境中不稳定,易被降解;在模拟人体肠环境中稳定,不易被降解。Objective To study the stability of HJC-1 protein and G6-EPSPS protein to digestion in simulated gastric fluid （SGF） and simulated intestinal fluid （SIF）. Methods The test model of stability of different protein to digestion in SGF and SIF was established. The component of SGF and SIF was based on the United States Pharmacopeia, the stability of HJC-l protein and G6-EPSPS protein were tested. The concentration of protein in SGF/SIF was 5.0 mg/ml and 2.0 mg/ml respectively. At intervals of 0 s, 15 s, 30 s, 1 min, 2 rain, 5 min, lO min, 20 rain, 30 rain and 60 min, the samples were taken out. According to the results of SDS-PAGE, the stability of two proteins to digestion in SGF/SIF was observed. Results The experiment showed that the HJC-1 protein was digested within 15 s in SGF/SIF. The G6-EPSPS protein was digested within 30 s in SGF and was stable within 60 rain in SIF. Conclusion HJC-1 protein and G6-EPSPS protein are instable in SGF, while, G6-EPSPS protein is stable in SIF.

关 键 词：HJC-1基因 G6-EPSPS基因 模拟胃液 模拟肠液 蛋白质 稳定性 

分 类 号：R994.6[医药卫生—毒理学]