转bar基因耐草胺膦油菜对大鼠的亚慢性毒性研究  

Study of subchronic toxicity of glyphosate resistant transgenic cole with bar gene in rats

作　　者：张静[1] 张静姝[1] 王静[1] 张力[1] 王晓军[1] 刘洪亮[1,2] 汪洋[1] 姜文玲[1] 卢长明[3] 

机构地区：[1]天津市疾病预防控制中心毒理室,天津300011 [2]天津医科大学公共卫生学院 [3]中国农业科学院油料作物研究所

出　　处：《环境与健康杂志》2012年第12期1077-1084,共8页Journal of Environment and Health

基　　金：国家科"十二五"基因重大专项(2008ZX08011-005)

摘　　要：目的观察转bar基因耐草胺膦油菜对大鼠的亚慢性毒性作用。方法将140只健康SPF级Wistar大鼠按体重随机分为7组,即对照(常规基础饲料)组和低(2.5%)、中(5.0%)、高剂量(10.0%)转基因组及低(2.5%)、中(5.0%)、高剂量(10.0%)亲本组,每组20只,雌雄各半。采用喂饲方式进行染毒,连续喂饲90 d。观察大鼠体重、血液学指标、血生化指标、各主要脏器重量及其系数,并进行病理学检查。结果各实验组动物的体重增长正常,血液学和血生化个别指标虽有统计学差异,但并无生物学意义。各剂量组大鼠心、肝、肾、肾上腺、脾、胸腺、睾丸的湿重及其脏器系数与对照组相比,差异均无统计学意义;且病理学检查未发现明显异常。结论转bar基因耐草胺膦油菜90 d喂养未对大鼠产生亚慢性毒性作用。Objective To investigate the subchronic toxicity of glyphosate resistant transgenic cole with bar gene on rats. Methods Wistar rats were randomly divided into seven groups by body weight, three dose groups of transgenic rape, three dose groups of non-transgenic groups and a basic diet control group. The rats were fed with diet for 90 consecutive days. In this period, animals were fed ad libitum. Food intakes and body weight were measured every week. In the middle and at the end of the feeding period, hematology and blood chemistry parameters were measured. At the end of the 90th day, routine pathological examination and organ coefficient for main viscera were carried out. Results Weight growth of each group animals was normal. Although some indexes of hematology and blood chemistry were significant different from transgenic cole group and non- transgenic cole group, all of the results were in the normal range which had been reported before. No obvious abnormality was found in pathological examination. Conclusion The results of the 90 days feeding test of transgenic rice in rats don＇t reveal any signs of toxic and adverse effects.

关 键 词：转bar基因耐草胺膦油菜 亚慢性毒性 安全性评价 

分 类 号：R994.6[医药卫生—毒理学]