基于普鲁士蓝修饰二氧化硅球腔阵列的葡萄糖传感器制备及应用  

Fabrication and Application of Glucose Sensor Based on Prussian Blue Modified Silicon Dioxide Cavities Array

作　　者：孙磊[1,2] 狄俊伟[1] 尹凡[3] 姚甜[2] 沈菁[2] 宗水珍[2] 

机构地区：[1]苏州大学材料与化学化工学部,江苏苏州215123 [2]常熟理工学院化学与材料工程学院,江苏常熟215500 [3]江苏省新型功能材料重点建设实验室,江苏常熟215500

出　　处：《常熟理工学院学报》2012年第10期60-64,共5页Journal of Changshu Institute of Technology

摘　　要：利用Langmuir-Blodgett技术和sol-gel法在氧化铟锡(ITO)电极上构建二氧化硅(SiO2)球腔微电极阵列.在此球腔微电极阵列上电沉积普鲁士蓝膜(PB),用于过氧化氢检测,并采用滴涂法将葡萄糖氧化酶(GOD)直接固定于PB/SiO2球腔微电极阵列上制得葡萄糖传感器(酶电极),酶电极对葡萄糖电流响应结果表明:葡萄糖浓度在4.7×10-6mol/L～3.8×10-3mol/L范围内呈线性关系,其检出限为1.35×10-6mol/L,该酶电极不受抗坏血酸、尿酸等电活性物质的干扰,稳定性较好.The silicon dioxide （SiO2） cavities microelectrode array was fabricated on the indium tin oxide （ITO） electrode, which was constructed by the Langmuir-Blodgett （LB） technology and sol-gel technology. The Prus- sian Blue （PB） film was fabricated on the SiO2 cavities array by electrodeposition technique, which was used to construct electrochemical sensor for the detection of hydrogen dioxide （H2O2）. A glucose biosensor was obtained by drop-coating glucose oxidase （GOD） in PB/SiO2 cavities array/ITO electrode directly .The resulting biosensor was highly sensitive to glucose with a linear calibration plot in the concentration range of 4.7×10^-6-3.8×10^-3 mol/L glucose, the detection limit 1.35×10^-6 tool/L glucose （S/N=3）. It is free of interference by ascorbic acid, uric ac.- id and other active substances .and also have good stability.

关 键 词：二氧化硅球腔 微电极阵列 普鲁士蓝 过氧化氢 葡萄糖传感器 

分 类 号：O657[理学—分析化学]