Preliminary Investigation on Somatic Embryogenesis from Immature Cotyledon Explants of Shea （Vitellaria paradoxa G,）  

作　　者：Paul Kwasi Krah Adu-Gyamfi Michael Teye Barnor Abu Mustapha Dadzie Samuel Lowor Stephen Yaw Opoku Kwabena Opoku-Ameyaw Matilda Bissah Francis Kwame Padi 

机构地区：[1]Department of Plant Breeding, Cocoa Research Institute of Ghana, Box 8, New-Tafo, Akim, Ghana [2]Department of Plant Breeding, Plant Genetic Resources Research Institute, Bunso, E/R Ghana

出　　处：《Journal of Agricultural Science and Technology(B)》2012年第11期1171-1176,共6页农业科学与技术（B）

摘　　要：Long juvenile phase and lack of effective protocols for large scale vegetative propagation are limitations to domestication and improvement of the shea tree. The present study seeks to develop a protocol for plant regeneration of shea （Vitellaria paradoxa） from immature cotyledon explants. Embryogenic callus cultures were induced on Murashige and Skoog medium （MS） containing 3% sucrose, 0.24% Phytagel, and various concentrations of 2,4-Dichlorophenoxyacetic acid （2,4-D） after four weeks of culture in darkness. Rates of embryogenic callus induction were significantly affected by the addition of 2, 4-D to the medium. Within 28 days of culture, the highest percentage of embyogenic calli （77.61%） occurred on MS media containing 0.45 ~tM of 2,4-D in the dark. Somatic embryos were obtained by culturing embryogenic callus （in the dark） on MS medium fortified with 3% sucrose, 0.24% phytagel and devoid of growth regulators. Culturing at 16 h photoperiod restricted both the induction of embryogenic calli cultures and somatic embryos. Somatic embryos germinated, developed shoots and rooted vigorously on MS medium devoid of growth regulators. Germinated plantlets were acclimatized, successfully.

关 键 词：Embryogenic callus MICRO-PROPAGATION Sapoteaceae shea tree somatic embryos. 

分 类 号：S565.403.5[农业科学—作物学] Q943.1[生物学—植物学]