四种植物提取物对大肠杆菌的抑菌效果研究  

Study on the Antibacterial Effect of the Extracts from 4 Species of Plants on Escherichia coli

作　　者：金兰梅[1] 伍清林[1] 马玉[1] 周玲玲[1] 傲国霞[1] 刘银燕[1] 曾疆雪[1] 

机构地区：[1]金陵科技学院动物科学与技术学院,江苏南京210038

出　　处：《家畜生态学报》2012年第6期72-77,共6页Journal of Domestic Animal Ecology

基　　金：2011年江苏省大学生实践创新训练计划项目(40700060);金陵科技学院教育教学改革研究项目(TY091007)

摘　　要：为充分利用当地资源和进一步开发植物药进行畜禽大肠杆菌病防治提供科学依据,采摘了本地生长的马鞭草、马齿苋、忍冬藤、蒲公英等四种植物,应用水提醇沉法制备提取液,用纸片扩散法和试管两倍稀释法对猪源、鸽源、牛源大肠杆菌进行体外抑菌效果试验,同时以小鼠为试验动物,检测四种植物提取液体内的抗菌作用。结果显示,四种植物对猪源大肠杆菌的抑菌圈在10～14mm之间,均为中敏;对鸽源大肠杆菌在10～22mm之间,马鞭草极敏,蒲公英高敏;对牛源大肠杆菌在7～14mm之间,马鞭草高敏,蒲公英、忍冬藤中敏,马齿苋低敏。马鞭草对猪源、鸽源大肠杆菌的MIC和MBC是31.25mg/mL,对牛源是62.5mg/mL;蒲公英对猪源、鸽源的大肠杆菌MIC和MBC是62.5mg/mL,对牛源是125mg/mL;马齿苋对猪源的MIC和MBC是62.5mg/mL和125mg/mL,对鸽源、牛源的MIC和MBC分别是125mg/mL和250mg/mL;忍冬藤对猪源、牛源的MIC和MBC分别是125mg/mL和250mg/mL,对鸽源MIC和MBC是62.5mg/mL和125mg/mL。小鼠体内抗菌作用提示,用蒲公英和马鞭草1.88g/kg、马齿苋和忍冬藤3.75g/kg进行预防,可使感染大肠杆菌的小鼠成活率为达100%。四种植物提取液对大肠杆菌体外抑菌和体内抗菌均有较好的效果,马鞭草效果最好。To prevent the spread of Escherichia coli in raised animals and invent new herbal medicine, the experiment adopted Verbena o f ficinalis , Portulace oleracea , Caules Lonicerae and Taraxacurn rnongon to extract the medicine. The experiment used dilute solution of the medicine on mice to determine the bac- teria＇s sensitivity. It showed the sensitivity of pig＇s E. coli to the drug is moderate and the range of antibac- terial diameters was 10-14 mm. The sensitivity of pigeon＇s E. coli to the drug was high and the range of antibacterial diameters was 10-22 mm. The sensitivity of cow＇s E. coli to the drug was high and the range of antibacterial diameters was 7-14 mm. The Verbena officinalis＇s MIC to pig＇s and cow＇s E. coli were 31.25 mg/mL,62.5 mg/mL. The Taraxacum mongon＇s MIC to pigs and cow＇s E. coli were 62.5 mg/mL, 125 mg/mL. The Taraxacum mongon＇s MIC to pig＇s and cow＇s E. coli were 62.5 mg/mL, 125 mg/mL. The Portulace oleracea＇s MIC to pigs and cow＇s E. coli were 62.5 mg/mL, 125 mg/mL, while MBC were 125 mg/mL, 250 mg/mL. The Caules Lonicerae＇s MIC to pig＇s and pigeon＇s E. coli were 125 mg/mL, 125 mg/mL, while MBC were 125 mg/mL, 250 mg/mL. The experiment on mice showed with Verbena officinalis and Taraxacum mongon 1.88 g/kg ,Portulace oleracea and Caules Lonicerae 3.75 g/kg, the survival rate of mice was 100%. Four plants were all effective to E. coli and Verbena officinalis had the best effect.

关 键 词：植物 大肠杆菌 抗菌试验 

分 类 号：S811.6[农业科学—畜牧学]