低铁环境对去势小鼠血清骨钙素及骨组织影响的初步研究  

A preliminary study on the influence of low-iron environment on serum osteocalcin and bone micro-structure in ovariectomized mice

作　　者：高超[1] 施勤[2] 贾鹏[1] 张增利[3] 徐又佳[1] 

机构地区：[1]苏州大学附属第二医院骨科,215004 [2]苏州大学骨科研究所 [3]苏州大学公共卫生学院卫生毒理研究室

出　　处：《中华内分泌代谢杂志》2012年第12期967-971,共5页Chinese Journal of Endocrinology and Metabolism

基　　金：基金项目：教育部博士点专项基金资助项目（20103201110020）;江苏省医学领军人才项目;苏州市科技发展计划项目（SYS201002）

摘　　要：目的观察去势小鼠在低铁环境下血清骨钙素及骨微结构的变化。方法24只12周龄C57BL／6小鼠，体重（19．5±0．5）g分为假手术组（SHAM）、模型组（OVX）、低铁组（OVX±DFO），每组8只。低铁组于卵巢切除后第2天腹腔注射甲磺酸去铁胺（DFO），剂量为30mg／kg，每周3次，共5周；模型组、假手术组均腹腔注射同等剂量0．9％生理盐水，共5周；第5周末取血后处死小鼠并分离子宫、左侧股骨；ELISA试剂盒检测血清骨钙素、血清铁蛋白；分析天平称子宫重量；显微CT扫描股骨末端并取目标区进行三维重建，获取骨小梁、骨皮质三维结构图像，进行松质骨、皮质骨定量分析。结果（1）血清骨钙素、血清铁蛋白：低铁组均显著低于模型组、假手术组（P〈O．01）；（2）松质骨、皮质骨骨密度：低铁组均显著低于模型组、假手术组（P〈0．01），骨小梁厚度、骨小梁间隔低铁组显著增加（P〈0．01），骨小梁数量、骨体积分数低铁组显著降低（P〈0．01）。结论一定剂量的DFO（30mg／kg）可以降低去势小鼠血清铁蛋白水平和骨组织形成指标。Objective To investigate the changes of serum osteocalcin and bone micro-structure in ovariectomized mice exposed to low-iron environment. Methods Twenty-four 12-week-old female C57BL/6 mice were divided equally into sham operation （SHAM） group, model（OVX） group, and low iron（OVX＋DFO） group. In low-iron group, deferoxamine （ DFO ） was injected 3 times per week for 5 weeks after operation ; the other groups were injected with the same dose of 0.9% normal saline for 5 weeks. The serum, left femur, uterus were harvested after five weeks of treatment. The serum osteocalcin and ferritin levels were measured by ELISA kit, the weight of the uterus was recorded by analytical balance. A high resolution micro-CT was used to scan the left femur for cortical bone and cancellous bone analysis. Results （ 1 ） The serum osteocalcin and serum ferritin levels in low-iron group were significantly lower than those in the other 2 groups （ P〈0.01 ） ; （ 2 ） Compared with the sham group and ovx group, there were significant decrease of the BMD ,BV/TV and Tb. N, but increase of Tb. Th and Tb. Sp in low-iron group （ P〈0.01 ）. Conclusion A certain dose of DFO （ 30 mg/kg） can decrease the serum ferritin levels as well as the bone formation index in ovariectomized mice.

关 键 词：低铁 骨质疏松症 骨钙素 铁蛋白 显微CT 

分 类 号：R580[医药卫生—内分泌]