脂联素球状结构域对胰岛素抵抗模型脂肪细胞及胰岛素信号转导的影响  

Effect of globular domain of adiponectin on glucose metabolism and insulin signal transduction in insulin resistant model adipocytes

作　　者：王彦[1] 陈思思[2] 廉如[2] 陈显久[3] 杨静[1] 

机构地区：[1]山西医科大学第一医院内分泌科,太原030001 [2]山西医科大学2004级七年制临床医学 [3]山西医科大学生物化学与分子生物学教研室

出　　处：《中华内分泌代谢杂志》2012年第12期1016-1019,共4页Chinese Journal of Endocrinology and Metabolism

基　　金：山西省科技攻关项目（20090321104）

摘　　要：将3T3-L1前脂肪细胞诱导分化为成熟的脂肪细胞，用软脂酸制备脂肪细胞胰岛素抵抗模型，不同浓度的脂联素球状结构域（globular domain of adiponectin，gAd）干预已经产生胰岛素抵抗的3T3-L1脂肪细胞，葡萄糖氧化酶法检测培养液中葡萄糖的消耗量，实时荧光定量PCR法检测胰岛素受体底物（1RS）-1、磷脂酰肌醇3激酶（P13K）、蛋白激酶B（PKB）基因水平的变化，Western印迹检测IRS-1酪氨酸磷酸化水平。结果显示，与对照组相比，各实验组葡萄糖消耗量均显著增加（P〈0．01），且随着gAd浓度的增加．葡萄糖消耗量也逐渐增加；500ng／mlgAd组及1000ng／mlgAd组IRS—1、P13K、PKB的mRNA表达均比对照组显著增加（P〈0．05）；同时，gAd可增加3T3-L1脂肪细胞胰岛素抵抗模型IRS-1酪氨酸磷酸化水平，且呈浓度依赖性．提示gAd能够促进3T3-LI脂肪细胞胰岛素抵抗模型葡萄糖的摄取，其机制可能与促进脂肪细胞胰岛素信号转导、改善胰岛素抵抗有关。Insulin resistance model of3T3-L1 adipocytes were prepared with plamotic acid. Adipocytes with generated insulin resistance were cultured with different concentrations of globular domain of adiponectin（ gAd ： 250, 500, 1 000 ng/ml）. The cell culture medium glucose content was detected with the glucose oxidase method, the mRNA expressions of insulin receptor substrate-I （ IRS-1 ） , phosphatidylinositol-3 kinase （ PI3 K） , and protein kinase B（PKB） were detected with real-time quantitative PCR method. The phosphorylation of IRS-I was detected by Western blot. Compared with the control group, the experimental group showed significantly increased glucose consumption （P 〈 0. 01 ）, and with the increasing gAd concentration, glucose consumption was gradually increasing. IRS-1 phosphorylation was increased gradually with the increasing concentration of gAd. These results suggest that gAd can promote glucose uptake by 3T3-L1 adipocyte model with generated insulin resistance. This may be correlated with promoting insulin signal transduction and improving insulin resistance in adipocytes.

关 键 词：脂联素球状结构域 3T3-L1脂肪细胞 胰岛素信号转导 胰岛素抵抗 

分 类 号：R587.1[医药卫生—内分泌]