Effect of Lichong Decoction on expression of IGF-I and proliferating cell nuclear antigen mRNA in rat model of uterine leiomyoma  

作　　者：Donghua Li Yalan Zhang Hongjuan Han Jianguo Geng Xiaolei Xie Jiubo Zheng Yasong Wang Xiaoli Zou 

机构地区：[1]School of Traditional Chinese Medicine,Capital Medical University [2]Beijing Maternal and Child Health Care Hospital [3]Beijing Obstetrics and Gynecology Hospital,Capital Medical University

出　　处：《Journal of Traditional Chinese Medicine》2012年第4期636-640,共5页中医杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.81073096);the Natural Science Foundation of Beijing City(No.7082015);the "Cultivation Plan for Youth Backbone Talents" Project(PHR 201008403) of Higher SchoolTalent Strong Education Plan

摘　　要：OBJECTIVE:To study the effect of Lichong Decoction(Lichong Decoction for strengthening anti-pathogenic Qi and eliminating blood stasis) on the expression of insulin-like growth factor-I(IGF-I) and proliferating cell nuclear antigen(PCNA) mRNA in a rat model of uterine leiomyoma.METHODS:Fifty female Wistar rats were randomized into a normal control group,model group,Lichong Decoction group,Guizhifuling Capsule(Capsule containing Cassia Twig and Poria) group,and Mifepristone group.The uterine leiomyoma model was established by peritoneal injections of exogenous estrogen and progesterone hormone.The ultrastructural changes in cells of rat uterine tissues were observed with transmission electron microscopy,and the expression of IGF-I and PCNA mRNA was detected by real-time fluorescent quantitative PCR.RESULTS:Following treatment,cells in the Lichong Decoction group appeared to be arranged normally,the cellular morphology were almost in a normal state,hyperplasia and hypertrophy of the chondriosome was reduced,collagen fibers were arranged in a regular manner,without obvious hyperplasia,and the expression of IGF-I and PCNA mRNA was significantly decreased compared with the model group(P<0.01).CONCLUSIONS:The effect of Lichong Decoction on uterine leiomyoma is related to its function in reducing the expression of IGF-I and PCNA mRNA.OBJECTIVE： To study the effect of Lichong Decoc- tion （Lichong Decoction for strengthening an- ti-pathogenic Qi and eliminating blood stasis） on the expression of insulin-like growth factor-I （IGF-I） and proliferating cell nuclear antigen （PCNA） mRNA in a rat model of uterine leiomyoma. METHODS： Fifty female Wistar rats were random- ized into a normal control group, model group, Li- chong Decoction group, Guizhifuling Capsule （Cap- sule containing Cassia Twig and Poria） group, and Mifepristone group. The uterine leiomyoma model was established by peritoneal injections of exoge- nous estrogen and progesterone hormone. The ul- trastructural changes in cells of rat uterine tissues were observed with transmission electron micros- copy, and the expression of IGF-I and PCNA mRNAwas detected by real-time fluorescent quantitative PCR. RESULTS： Following treatment, cells in the Lichong Decoction group appeared to be arranged normal- ly, the cellular morphology were almost in a normal state, hyperplasia and hypertrophy of the chondrio- some was reduced, collagen fibers were arranged in a regular manner, without obvious hyperplasia, and the expression of IGF-I and PCNA mRNA was significantly decreased compared with the model group （P〈0.01）. CONCLUSIONS： The effect of Lichong Decoction on uterine leiomyoma is related to its function in re- ducing the expression of IGF-I and PCNA mRNA.

关 键 词：LEIOMYOMA Insulin-like growth factor I Proliferating cell nuclear antigen  Lichong Decoction 

分 类 号：R285[医药卫生—中药学]