马钱子生物碱组分的提取和分离制备  

Extraction and Isolation of Alkaloids from Strychni Semen

作　　者：蒋莹莹[1] 陈海波[1] 李玉燕[1] 朱敏[1] 马凤森[1] 

机构地区：[1]浙江工业大学,杭州310014

出　　处：《中国现代应用药学》2012年第12期1094-1097,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：浙江省自然科学基金项目(Y2111194);浙江省重大科技专项重点社会发展项目(2010C13014)

摘　　要：目的建立马钱子生物碱组分的超声提取方法,研究其分离纯化工艺。方法以马钱子碱和士的宁含量为指标,采用均匀设计筛选马钱子生物碱的最佳超声提取工艺,并采用高速逆流色谱法分离其生物碱组分。结果最佳提取工艺为10倍量50%乙醇溶液(pH=5),在600 W功率下超声60 min;应用高速逆流色谱法,以正己烷-乙酸乙酯-甲醇-水(3∶5∶3∶5)为溶剂体系,分离得到纯度为94.1%的马钱子碱组分和纯度为92.5%的士的宁组分。结论本实验优选出的提取工艺稳定、经济、可行,高速逆流色谱适用于马钱子生物碱的分离纯化。OBJECTIVE To establish an efficient method for extracting and isolating the alkaloids from Strychni Semen. METHODS With the content of brucine and strychnine as index, the ultrasonic assisted extraction process was optimized by uniform design method, and high-speed counter-current chromatography（HSCCC） was employed to separate the alkaloids. RESULTS The optimum condition of extraction was as follows： ratio of solid to liquid was 1 ： 10, ethanol concentration was 50%（pH=5）, ultrasonic power was 600 W, and the extraction time was 60 min; the two-phase solvent system of HSCCC composed of n-hexane-ethyl acetate-methanol-water（3 ： 5 ： 3 ： 5） was selected, brucine with the purity of 94.1% and strychnine with the purity of 92.5% were obtained. CONCLUSION The optimum extraction method is economical and feasible. HSCCC is suitable for the isolation of alkaloids from Strychni Semen.

关 键 词：马钱子 生物碱 超声提取 高速逆流色谱 

分 类 号：R284.1[医药卫生—中药学] R917.101[医药卫生—中医学]