比格犬胸部多发伤出血性休克模型建立  

A Model of Traumatic Hemorrhagic Shock with Multiple Thoracic Injuries in Beagle Dogs

作　　者：孟辉[1] 郑军 李庆新[1] 洪波 张世范[1] 李乃斌[1] 高明[1] 杨俊峰 王海芳 

机构地区：[1]兰州军区兰州总医院胸外科,730050 [2]广州军区广州疗养院

出　　处：《医学研究杂志》2012年第12期51-55,共5页Journal of Medical Research

基　　金：甘肃省科技支撑计划基金资助项目(1104FKCA106);甘肃卫生行业科研计划项目(GSWST09-19)

摘　　要：目的建立一种胸部多发伤失血性休克大动物模型,满足一线与后续救护实验研究需要。方法普通级比格犬18只随机分为微创血气胸、失血休克组(A组,n=6),多根多段肋骨骨折、创伤性血胸、张力性气胸、失血性休克组(B组,n=12),后者又分为自体血回输组(B1组,n=6)、非自体血回输组(B2组,n=6)。A、B2组常规液体复苏,B1组在液体复苏的基础上用自制救护装置自体输血复苏。观察动物的存活率、生理指标、血常规、凝血、动脉血气各项参数。结果 A、B1组动物全部成活(6/6,12/18),B2组半数死亡(3/6)。与A组相比,伤后B1组RBC、HGB、HCT、PaO2、氧合指数有显著性差异(P<0.05),明显优于B2组;伤后4～8h,B2组RBC、HGB、HCT、PaO2、氧合指数降低较为明显,与B1组相比有显著性差异(P<0.05)。结论本法制作的胸部多发伤失血性休克模型成功率高,可重复性强。Objective To establish a model of traumatic hemorrhagic shock with multiple thoracic injuries in Beagle dogs for the ex- perimental study of the rescuing effect of the first - line and follow - up comprehensive rescue devices. Methods Eighteen beagle dogs of common grade were randomly divided into two groups, which included mini -traumatic shock and hemopneumothorax group（ group A, n = 6） , multiple fractured ribs and traumatic hemothorax and tension pneumothorax and hemorrhagic shock group（ group B, n = 12） , and then the latter was dirided into with autologous blood transfusion group （group B1 , n = 6） and without autologous blood transfusion group （group B2 , n = 6）. Routine liquid resuscitation were applied in group A and B2 , and autotransfusion of self - made serial first - aid life supporting devices in group B1. Animal survival rate, physiological index, and parameters of routine blood test, blood gas and coagulation were ob- served. Results The survival rate in group A and B1 was 100% but it was 50% in group B2. Compared with the control group A, red blood cell（ RBC）, hemoglobin（ HGB）, hematocrit（ HCT）, arterial partial pressure of oxygen（ PaO2 ） and oxygenation index （P 〈 0.05）, the configuration distortion indexes after trauma in the experimental group B1 were better than B2. Compared with the control group B1 ,the indexes of RBC,HGB, HCT,PaO2 and oxygenation index were brightly reduced and had significant differences in B2after trauma. Conclu- sion It is successful and repeatable that the model of traumatic hemorrhagic shock with multiple thoracic injuries in Beagle dogs is estab- lished by this way. It is suited for experimental study of the serial first - aid life supporting devices.

关 键 词：创伤 失血性休克 血气胸 比格犬 动物模型 

分 类 号：R557[医药卫生—血液循环系统疾病]