Msi1 siRNA对人结肠癌SW-480细胞迁移和侵袭能力的影响  

Impact of Msi1 siRNA on the Migration and Invasion of SW-480 Cell Line

作　　者：魏建国[1] 孙爱静[1] 赵芳 

机构地区：[1]绍兴市人民医院病理科,312000 [2]绍兴县中心医院妇产科,312030

出　　处：《医学研究杂志》2012年第12期73-76,共4页Journal of Medical Research

基　　金：浙江省医学重点学科建设计划(GJSX-010-004)

摘　　要：目的探讨siRNA沉默Msi1基因表达对人结肠癌SW-480细胞迁移和侵袭能力的影响及其作用机制。方法设计合成特异性Msi1 siRNA序列,转染人结肠癌SW-480细胞;利用Western blotting检测Msi1和MMP-9蛋白的表达;细胞免疫化学检测MMP-9蛋白的表达;划痕实验观察Msi1 siRNA对细胞迁移能力的影响;Transwell实验观察其对细胞侵袭能力的影响。结果 Msi1 siRNA能有效抑制人结肠癌SW-480细胞中Msi1蛋白的表达;并且使其迁移和侵袭能力明显下降,和对照组相比有统计学意义(P<0.05),同时MMP-9在蛋白表达水平明显降低。结论沉默Msi1基因后可以显著抑制人结肠癌SW-480细胞的迁移和侵袭能力,MMP-9基因表达下降可能参与这一过程。Objective To investigate the effect of silencing Msil gene with siRNA on cell migration and invasion of SW - 480 cell line. Methods Msil -specific siRNA was designed and synthesized. Small interfering RNA was transfected into SW- 480 cells to knockdown the Msil expression. Protein levels of Msil and MMP9 were detected by Western blot. Protein levels of MMP9 were detected by immunohistochemical assay. Cell migration and invasion were detected by wound healing assay and transwell assay. Results RNA inter-ference efficiently suppressed the Msil expression in SW - 480 cell line after SW - 480 cells transfected with Msil siRNA. Wound Healing assay and Transwell assay showed that Msil gene was interfered. SW-480 cells significantly reduced migration and invasion（ P 〈 0.05 ）, and the expression of MMP9 protein levels decreased. Conclusion Downregulation of Msil leads in to significant inhibition of cell migra- tion and invasion in the SW -480 cell line. The reduction of MMP -9 in protein expression may be involved in this process.

关 键 词：结肠癌 Msi1 RNAI 迁移 侵袭 

分 类 号：R977.21[医药卫生—药品]