化感物质肉桂酸乙酯对蛋白核小球藻生长及生理特性的影响  

Effects of Allelochemicals Ethyl Cinnamate on the Growth and Physiological Characteristics of Chlorella pyrenoidosa

作　　者：高李李[1] 郭沛涌[1] 苏光明[1] 魏燕芳[1] 

机构地区：[1]华侨大学化工学院环境科学与工程系,厦门361021

出　　处：《环境科学》2013年第1期156-162,共7页Environmental Science

基　　金：国家自然科学基金项目(20777021);教育部科学技术研究重点项目(210253);福建省自然科学基金项目(2010J01043);华侨大学科研基金项目(10HZR11)

摘　　要：探讨了肉桂酸乙酯对蛋白核小球藻生长及生理特性的影响,从叶绿素a含量,抗氧化系酶活性,活性氧自由基(ROS)水平,丙二醛(MDA)含量以及光合活性的变化研究了其抑藻的生理生化机制.结果表明,肉桂酸乙酯对蛋白核小球藻具有快速抑制效应,随着浓度的增加抑制效果越明显.其96 h半效应浓度EC50值为5.45 mg·L-1.肉桂酸乙酯引起细胞内叶绿素a含量下降,活性氧(ROS)的过度累积和MDA含量的增加.由此,说明肉桂酸乙酯通过引起活性氧的过度产生,导致膜脂过氧化,引起藻细胞膜系统受损,代谢发生紊乱.为了清除ROS,细胞通过提高抗氧化系酶的活性来保护其免受氧化损伤.肉桂酸乙酯对蛋白核小球藻细胞光合系统Ⅱ的最大光化学量子产量和潜在活性具有一定的急性毒性效应,但随着时间的延长藻细胞可以通过自身调节在一定程度上恢复其光合活性.The effects of ethyl cinnamate on the growth and physiological characteristics of Chlorella pyrenoidosa were studied.The allelopathic mechanisms were explored,from views of chlorophyll a content,antioxidant enzyme activities,reactive oxygen species（ROS） level,malondialdehyde（MDA） content and photosynthetic activity.The results revealed that ethyl cinnamate had acute inhibitory effects on the growth of Chlorella pyrenoidosa,and the inhibited degree tended to increase with increased concentrations.The effective concentration causing a 50% inhibition at 96 h was 5.45 mg·L-1.Ethyl cinnamate induced the decrease of chlorophyll a,the over-accumulation of ROS and the increase of MDA.Therefore,it suggested that ethyl cinnamate could lead to the damage of cell membrane system and metabolic disorder through inducing lipid peroxidation via initiating ROS overproduction.And for scavenging ROS,the algae cells were protected from oxidative damages through increasing the activity of antioxidant enzymes.The results demonstrated ethyl cinnamate had acute inhibition to the maximum quantum yield and the potential activity of photosystem Ⅱ of Chlorella pyrenoidosa,however,the photosynthetic activity could recover to some extent through self-regulation after some time.

关 键 词：肉桂酸乙酯 蛋白核小球藻 生理特性 光合活性 除藻剂 

分 类 号：X52[环境科学与工程—环境工程]