偏硅酸钠对氧化低密度脂蛋白刺激iNOS表达的抑制作用  

Suppression of oxidized low density lipoprotein-induced inducible nitric oxide synthase expression by Na_2SiO_3

作　　者：黄伟[1] 侯建明[2] 李丽纬[3] 甄艳军[2] 李志永[2] 

机构地区：[1]枣庄职业学院 [2]河北医科大学西校区基础课教学部,河北石家庄050091 [3]邢台市人民医院检验二科

出　　处：《中国老年学杂志》2012年第24期5452-5454,共3页Chinese Journal of Gerontology

基　　金：河北省科技厅科技支撑计划项目(08276101D-103)

摘　　要：目的观察偏硅酸钠对氧化低密度脂蛋白(ox-LDL)刺激的ECV-304细胞诱导型一氧化氮合酶(iNOS)表达的抑制作用及机制。方法 ECV-304细胞分成5组,对照组以及oxLDL组和加偏硅酸钠干预3个组(68.5、34.2、17.1 mg/L组),给予ox-LDL刺激后比较各组细胞培养液NO浓度、细胞内NOS、iNOS活性和蛋白以及mRNA,同时检测LOX-1和NOX4的mRNA。结果与oxLDL组比较,加偏硅酸钠干预的3个组NO浓度上升、NOS活性提高(P<0.01);iNOS活性、mRNA水平、蛋白量均增加(P<0.01)。加偏硅酸钠的各组LOX-1和NOX4的mRNA也比oxLDL组显著下降。结论偏硅酸钠能抑制ox-LDL刺激的ECV-304细胞iNOS基因表达,其作用机制与下调LOX-1和NOX4表达有关。Objective To observe the effect and mechanism of Na/SiO3 on ECV-304 cell-line inducible nitric oxide synthase （iN- OS） expression. Methods ECV-304 ceU-lines were divided into control, oxidant low-density lipoproteins （ox-LDL） , 68.5 rag, 34.2 mg and 17.1 mg Na2SiO3 groups. ECV-304 cell lines were exposed to ox-LDL and NazSiO3 , and then nitric oxide （NO） concentration, nitric oxide synthase （NOS） and inducible NOS （iNOS） activities and protein and mRNA in the culture fluid were measured. LOX-1, NOX4 mRNA in ECV-304 were examined by RT-PCR, the expression of iNOS protein was examined by western blotting. Results Compared with oxLDL group, all NazSiO3 groups had a significantly higher NO concentrations, and NOS activities were increased. Intracellular activities of iNOS and iNOS mRNA and protein levels were dramatically decreased by Na2SiO3 treatment, compared with ox-LDL group, activities of iNOS decreased to 55.6%, iNOS mRNA levels decreased to 66. 5%, iNOS protein levels decreased to 37.6%. LOX-1 mRNA and NOX4 mRNA levels were also decreased significantly. Conclusions Na2SiO3 can inhibit the ox-LDL induced expression of iNOS gene and the effect is possible related to inhibit LOX-1 and NOX4 expression.

关 键 词：偏硅酸钠 诱导型一氧化氮合酶 NO LOX-1 NOX4 

分 类 号：R543.5[医药卫生—心血管疾病]