心肌钙蛋白T单克隆抗体制备及应用  

Preparation of monoclonal antibodies against troponin T and its clinical application in acute myocardial infarction

作　　者：张琳[1] 游上游[2] 黄亦钧 胡远贵[1] 黄巍[2] 杨军[1] 

机构地区：[1]武汉市第六医院检验科,430016 [2]武汉市医学科学研究所

出　　处：《中华检验医学杂志》2000年第3期163-165,共3页Chinese Journal of Laboratory Medicine

摘　　要：目的　人心肌肌钙蛋白T单克隆抗体制备及在心肌梗死诊断中的初步应用。方法　在研究心肌特异性肌钙蛋白T(cTnT)提纯的基础上成功地制备出 3株特异性抗cTnT单克隆抗体 ,用其中的 2株Mcab建立了检测cTnT双单克隆抗体的夹心ELISA法。结果　对 30名健康献血员和 32例心肌梗死患者血中cTnT水平进行临床检测 ,正常人血清cTnT浓度平均为 (0 .12 7± 0 .0 84) μg L ,最低为 0 ,最高为 0 .42 μg L ,以血清cTnT >0 .40 μg L为诊断心肌梗死的临界值。 32例患者血清cTnT值平均为 (2 .10 8± 1.974) μg L ,患者血清cTnT分别用BM试剂盒及ELISA法检测 ,经统计学处理 ,二者相关系数为 0 98,符合临床检测要求。结论　该法灵敏度、准确度和精确度较高 。Objectives To preparae monoclonal antibodies against troponin T and study its clinical application in acute myocardial infarction.Methods Based on purification of human cTnT, three lines of monoclonal antibodies (McAbs) were prepared by hybridoma technique, and two McAbs Sandwich ELISA was estalished.Results The results suggest that serum cTnT concentration of 30 mormal donors, 32 patients with acute myocardial infarction was (0.127±0.084) μg/L (0～0.420 μg/L) and (2.108±1.974) μg/L respectively. Compared with B M cTnT kit (ELISA), the correlation coefficient of two methods was 0.98, which illustrates that the sandwich ELISA established is suitable in clinical determination.Conclusion cTnT is highly sensitive and accurate.

关 键 词：肌钙蛋白T 急性心肌梗塞 单克隆抗体 

分 类 号：Q785[生物学—分子生物学]