三七促进家兔慢性硬膜下血肿吸收的实验研究  

Experimental study of panax notoginseng in promoting the absorption of chronic subdural hematoma in rabbits

作　　者：邢海亭 施宏志 王超云[2] 姜文国[2] 杨茗[1] 

机构地区：[1]山东烟台市烟台山医院神经外科,264002 [2]滨州医学院药学院

出　　处：《中华神经外科杂志》2012年第12期1284-1287,共4页Chinese Journal of Neurosurgery

基　　金：国家自然科学基金（30901979）;山东高校科技计划（J09LF58）;烟台市科学技术发展计划（2011233）

摘　　要：目的观察三七对实验性慢性硬膜下血肿（CSDH）的促吸收效应。方法硬膜下少量多次注血法制备家兔CSDH模型，观察三七对血肿体积的影响；HE染色观察血肿外膜病理组织学特征；透射电镜和硝酸镧示踪法观察血肿外膜血管超微结构及通透性。结果三七组血肿液完全吸收或仅少量残余，与模型组相比差异有统计学意义（P〈0．01）；三七组血肿外膜炎性反应较模型组轻而纤维组织增生相对成熟；模型组毛细血管结构功能缺损，而三七组血管内皮及基膜完整连续，血管周围组织中镧颗粒沉积少。结论三七能加快CSDH血肿吸收速度，此效应可能与其减轻血肿外膜炎性反应、降低外膜新生血管通透性有关。Objective To observe the efficacy of Panax notoginseng in promoting the absorption of chronic subdural hematoma in rabbits. Methods A stable pathological animal model similar to CSDH in humans was established using a small number of subdural injections of blood through a subdural pre - catheter in rabbits. Panax notoginseng powder was administrated by lavage for six days. The volume of subdural hematoma in each group was observed. The pathological features of the hematoma outer membranes were observed via hematoxylin and eosin staining. The ultrastructures of the outer membranes were observed with transmission electronic microscopy. The permeability of outer membrane and neovascnlarization were detected with lanthanum nitrate. Results The group administrated with Panax notoginseng powder had a significantly lower volume of residual subdural hematoma compared with the model group （ P 〈 0. 01 ）. The dura mater associated with hematomas showed reactive thickening in both groups. The inflammatory reaction and fibrous tissue proliferation in the hematoma outer membrane of Panax notoginseng group were less than the model group. The structure and function of new capillaries in the outer membrane of the model group were defected, which resulted in red blood cells escaping outside the vessel and lanthanum granules deposited in the extracellular space. However, in Panax notoginseng group, the new capillaries developed well, endothelial cells and basement membranes were integrated and connected closely, and less lanthanum granules were found in the extracellular space. Conclusions Panax notoginseng could effectively promote the absorption of subdural hematoma or fluid. This might have resulted from relieving inflammation and reducing new capillary permeation in the outer membrane of a hematoma capsule, which may accelerate the physiopathologic process and thus promote the recovery process from pathological states to normal.

关 键 词：慢性硬膜下血肿 三七 兔 

分 类 号：R651.15[医药卫生—外科学]